DNA methylation, ageing and ribosomal genes activity

DNA methylation, ageing and ribosomal genes activity

Autor Carvalho, C. V. de Google Scholar
Payao, SLM Google Scholar
Smith, M. D. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo DNA methylation is an epigenetic mechanism controlling of gene expression. We studied the effect of a demethylation agent 5-azacytidine, on ribosomal gene activity observed by Nucleolus Staining Giemsa in the chromosomes of eight Alzheimer's disease patients, eight elderly healthy individuals and eight young healthy controls. Metaphase cells were obtained from lymphocyte cultures. All the acrocentric chromosome pairs were analyzed using G banding followed by Nucleolus Staining Giemsa banding in the same cell. the Alzheimer's disease patients and elderly healthy control did not show a variation in the transcriptional activity of ribosomal genes with or without 5-azacytidine treatment, whereas the young control group showed an increase in this activity with the treatment.
Palavra-chave ageing
ribosomal genes
Alzheimer's disease
DNA methylation
nucleolus organizer region
Idioma Inglês
Data de publicação 2000-01-01
Publicado em Biogerontology. Dordrecht: Kluwer Academic Publ, v. 1, n. 4, p. 357-361, 2000.
ISSN 1389-5729 (Sherpa/Romeo, fator de impacto)
Publicador Kluwer Academic Publ
Extensão 357-361
Fonte http://dx.doi.org/10.1023/A:1026542618182
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000166618300007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26225

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