Multiple pilocarpine-induced status epilepticus in developing rats: A long-term behavioral and electrophysiological study

Multiple pilocarpine-induced status epilepticus in developing rats: A long-term behavioral and electrophysiological study

Autor Santos, N. F. Google Scholar
Marques, R. H. Google Scholar
Correia, L. Google Scholar
Sinigaglia-Coimbra, Rita Autor UNIFESP Google Scholar
Calderazzo, Lineu Autor UNIFESP Google Scholar
Sanabria, ERG Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Purpose: Animal models are useful for the study of status epilepticus (SE)-induced epileptogenesis and neurological sequelae, especially during early brain development. Here. we show several permanent abnormalities in animals subjected to multiple SE during early development.Methods: Wistar pup rats (7 to 9 days old) were subjected to three consecutive episodes of SE induced by systemic pilocarpine injections. To study the long-lasting consequences of early-induced SE, chronic electroencephalographic recordings were made from the hippocampus and cortex and several behavioral tests (inhibitory step-down avoidance, rota-rod, open field, elevated plus-maze, and Skinner box) were performed at postnatal days 30 to 90. We also investigated in vitro electrophysiological responses of the CA1 area using extracellular recordings in hippocampal slices. A histological analysis was done using cresyl violet staining 24 hours and several months after SE induction. Apoptotic cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL staining) 24 hours after the last SE episode.Results: Electloencephalographic recordings from 30- to 90-day-old rats that had been subjected to multiple SE episodes in early life showed marked changes compared with those from nontreated controls. These included frequent episodes of continuous complex spiking activity and high-voltage ictal discharges, with a small percentage of these rats presenting spontaneous behavioral seizures. These animals also presented evidence of severe cognitive deficit in adulthood. in vitro, a persistent hyperexcitability of the CAI area was detected in experimental animals. Histological analysis of the brains did not reveal any major long-term pathological changes. Nevertheless, an increased number of TUNEL-positive nuclei were present in some animals in both the hippocampus and the thalamus.Conclusions: These data show persistent abnormalities in animals subjected to multiple SE episodes during early postnatal development. SE may result in important plastic changes in critical periods of brain maturation leading to long-lasting epileptogenesis, as manifested by electrogaphic epileptiform discharges, behavioral deficits, and in vitro hyperexcitability of hippocampal networks.
Palavra-chave development
epilepsy
hippocampus
pilocarpine
rat
Idioma Inglês
Data de publicação 2000-01-01
Publicado em Epilepsia. Philadelphia: Lippincott Williams & Wilkins, v. 41, p. S57-S63, 2000.
ISSN 0013-9580 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott Williams & Wilkins
Extensão S57-S63
Fonte http://dx.doi.org/10.1111/j.1528-1157.2000.tb01558.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000089156500012
Endereço permanente http://repositorio.unifesp.br/handle/11600/26206

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