Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase

Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase

Autor Ribeirao, Marcelo Autor UNIFESP Google Scholar
Pereira-Chioccola, Vera Lucia Autor UNIFESP Google Scholar
Renia, L. Google Scholar
Fragata, A. A. Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Rodrigues, Mauricio Martins Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Pazzanese Cardiol Estado São Paulo
Univ Paris 05
Resumo Infective forms of Trypanosoma cruzi, the parasite that causes Chagas' disease, express on their surface an enzyme denominated trans-sialidase (TS). the present study was designed to evaluate the naturally acquired immune responses to a bacterial recombinant protein representing the catalytic domain of TS in chronically infected chagasic individuals. the cellular immune response was measured by in-vitro T-cell proliferation and by interferon (INF)-gamma, interleukin (IL)-4 and IL-10 production in response to a whole-parasite homogenate and the recombinant protein. the peripheral blood mononuclear cells of 78.6% of 28 chagasic patients responded to the recombinant protein as estimated by T-cell proliferation. With respect to cytokine production, 88% of the cells of the chagasic individuals produced IFN-gamma on stimulation with the recombinant protein. in contrast, IL-4 or IL-10 were minimally produced in response to TS. the cellular immune response was specific because most healthy individuals never exposed to T. cruzi failed to react with this recombinant protein. the plasma of 71.4% or 100% of chagasic patients had Ige antibodies as determined by ELISA or by the presence of TS inhibitory antibodies, respectively. We conclude that the catalytic domain of TS is recognized by IFN-gamma producing type I cells and antibodies in a large proportion of patients infected with T. cruzi.
Palavra-chave Trypanosomes
sialidases
cell-mediated immune response
Idioma Inglês
Data de publicação 2000-01-01
Publicado em Parasite Immunology. Oxford: Blackwell Publishing Ltd, v. 22, n. 1, p. 49-53, 2000.
ISSN 0141-9838 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Publishing Ltd
Extensão 49-53
Fonte http://dx.doi.org/10.1046/j.1365-3024.2000.00260.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000085737500007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26200

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