Autoantibodies to DEK oncoprotein in human inflammatory disease

Autoantibodies to DEK oncoprotein in human inflammatory disease

Autor Dong, X. W. Google Scholar
Wang, J. S. Google Scholar
Kabir, F. N. Google Scholar
Shaw, M. Google Scholar
Reed, A. M. Google Scholar
Stein, L. Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Trevisani, Virgínia Fernandes Moça Autor UNIFESP Google Scholar
Miller, M. L. Google Scholar
Fujii, T. Google Scholar
Akizuki, M. Google Scholar
Pachman, L. M. Google Scholar
Satoh, M. Google Scholar
Reeves, W. H. Google Scholar
Instituição Univ N Carolina
Universidade Federal de São Paulo (UNIFESP)
Northwestern Univ
Keio Univ Hosp
Resumo Objective. To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA),Methods. Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation Studies using baculovirus-expressed human DEK.Results. Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21), Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. in general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.Conclusion. Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Th1 cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset.
Idioma Inglês
Data 2000-01-01
Publicado em Arthritis and Rheumatism. Philadelphia: Lippincott Williams & Wilkins, v. 43, n. 1, p. 85-93, 2000.
ISSN 0004-3591 (Sherpa/Romeo, fator de impacto)
Editor Lippincott Williams & Wilkins
Extensão 85-93
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000084782700011

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