Identification, sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single nucleocapsid baculovirus

Identification, sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single nucleocapsid baculovirus

Autor Chen, X. W. Google Scholar
Ijkel, WFJ Google Scholar
Dominy, C. Google Scholar
Zanotto, PMD Google Scholar
Hashimoto, Y. Google Scholar
Faktor, O. Google Scholar
Hayakawa, T. Google Scholar
Wang, C. H. Google Scholar
Prekumar, A. Google Scholar
Mathavan, S. Google Scholar
Krell, P. J. Google Scholar
Hu, Z. H. Google Scholar
Vlak, J. M. Google Scholar
Instituição Agr Univ Wageningen
Chinese Acad Sci
Univ Guelph
Universidade Federal de São Paulo (UNIFESP)
Kyoto Inst Technol
Hebrew Univ Jerusalem
Univ Calif Davis
Natl Taiwan Univ
Madurai Kamaraj Univ
Resumo The baculovirus late expression factor 2 (LEF-2) is involved in DNA replication, and most likely function as a primase processivity factor. Lef-2 genes have been found in multinucleocapsid nucleopolyhedroviruses (MNPVs) and in granuloviruses (GVs), but not yet in single-nucleocapsid NPV (SNPV). Here, a lef-2 gene homolog was identified from SNPV of Helicoverpa armigera (HearNPV). the open reading frame of the HearNPV lef-2 gene is 696 nucleotides long, encoding a putative protein of 232 amino acids with an M-r of about 26 kDa. the 5'-noncoding region contains two early (CAGT) consensus motifs for transcription initiation and three TATA boxes. Lef-2 transcripts started at a C, 29 nucleotides upstream of a putative translational start. A putative polyA signal, AATAAA, was found 76 nucleotides downstream of the translation stop codon. the HearNPV lef-2 gene has a low but significant degree of amino acid sequence identity (30%) to the lef-2 genes of 15 other baculoviruses of which nine were newly determined. the N-terminal half of the LEF-2 proteins: contains one (I) and the C-terminal half two (II and III) conserved domains. Sixteen amino acids are absolutely conserved in those LEF-2 investigated and are probably critical for LEF-2 function. A phylogenetic tree of 16 baculovirus LEF-2 proteins was constructed by using maximum parsimony analysis and appeared to be comparable: to a tree for ecdysteroid UDP-glucosyl transferases (Chen et al., 1997a). the genomic location of the lef-2 genes relative to polyhedrin/granulin and the clade structure of the gene trees suggest that genome organization and gene phylogeny are useful parameters to study the evolutionary history of baculoviruses. These two independent approaches also give a more complete picture of the ancestral relationship among baculovirus. (C) 1999 Elsevier Science B.V. All rights reserved.
Palavra-chave baculovirus
Helicoverpa armigera
lef-2 gene
phylogeny
sequence analysis
Idioma Inglês
Data de publicação 1999-12-01
Publicado em Virus Research. Amsterdam: Elsevier B.V., v. 65, n. 1, p. 21-32, 1999.
ISSN 0168-1702 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 21-32
Fonte http://dx.doi.org/10.1016/S0168-1702(99)00097-0
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000084175700004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26194

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