Comparison of human and porcine tissue kallikrein substrate specificities

Comparison of human and porcine tissue kallikrein substrate specificities

Autor Del Nery, E. Google Scholar
Chagas, JR Google Scholar
Juliano, M. A. Google Scholar
Juliano, L. Google Scholar
Prado, E. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Little is known about the species specificity of tissue kallikrein-kininogen interaction since the kinetic parameters for Lys-bradykinin release from kininogen by tissue kallikreins from different animal species have not been reported. We have now determined the kinetic parameters for hydrolysis by human and porcine tissue kallikrein, hK1 and pK1, respectively (Berg et al., 1992) of two series of intramolecularly quenched fluorogenic peptides having the sequences that flank the scissile Arg-Ser or Met-Lys bond in human and bovine kininogen. Results have shown that peptides having sequences from human kininogen are better substrates for hK1 and peptides derived from bovine kininogen are better substrates for pK1. Kinetic data for hydrolysis of the Arg-Ser bond showed that differences in the interaction of residue(s) in positions P-2'-P-10' contribute to the efficiency of the cleavage and may be responsible for differences in their susceptibilities to the two kallikreins. Significant variations in the kinetic data were observed for the hydrolysis of the Met-Lys bond in substrates with an N-terminal extension at sites P-3-P-9. the highest k(cat)/K-m value in the hydrolysis of Abz-[Gln(370)-Gln(381)]-bkng-EDDnp by pk1 demonstrates an important interaction of subsites S-5-S-4 with Gln and Thr residues in the bovine kininogen segment. A Gln(370)-Gln(391) bovine kininogen fragment used to study the cleavage of both Met-Lys and Arg-Ser bonds in the same molecule confirmed the importance of an extended interaction site for species specificity among tissue kallikreins. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
Palavra-chave species specificity of tissue kallikreins
Idioma Inglês
Data de publicação 1999-12-01
Publicado em Immunopharmacology. Amsterdam: Elsevier B.V., v. 45, n. 1-3, p. 151-157, 1999.
ISSN 0162-3109 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 151-157
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000084080100025
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