A double headed serine proteinase inhibitor - human plasma kallikrein and elastase inhibitor - from Boophilus microplus larvae

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dc.contributor.author Tanaka, A. S.
dc.contributor.author Andreotti, R.
dc.contributor.author Gomes, A.
dc.contributor.author Torquato, RJS
dc.contributor.author Sampaio, M. U.
dc.contributor.author Sampaio, CAM
dc.date.accessioned 2016-01-24T12:30:57Z
dc.date.available 2016-01-24T12:30:57Z
dc.date.issued 1999-12-01
dc.identifier http://dx.doi.org/10.1016/S0162-3109(99)00074-0
dc.identifier.citation Immunopharmacology. Amsterdam: Elsevier B.V., v. 45, n. 1-3, p. 171-177, 1999.
dc.identifier.issn 0162-3109
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26186
dc.description.abstract Preying on cattle, the hard tick Boophilus microplus causes heavy economical losses to Brazil. Tick proteins are a good target to be used as tools for tick control. Serine protease inhibitors from B. microplus larvae (BmTI) were preliminarily characterized. One-week-old larvae were the source of a 2% protein solution in 5 mM Tris-HCl, 20 mM NaCl, pH 7.4. the inhibitors were purified by affinity chromatography on trypsin-Sepharose, and ion-exchange chromatography on Resource Q column, and they separated in two major active peaks, corresponding to 10-kDa and 18-kDa proteins (BmTI-B and BmTI-A, respectively). Both purified proteins inhibited trypsin with K-i of 0.3 and 3.0 nM, respectively, but only the 18-kDa protein inhibited elastase (K-i 1.4 nM) and plasma kallikrein (K-i 120 nM). BmTI-A did not change prothrombin time (PT) and thrombin time (TT), but it increased activated partial thromboplastin time (APTT) was dose-dependent. the partial amino acid sequence indicated that BmTI-A belongs to the bovine pancreatic trypsin inhibitor (BPTI)-Kunitz type inhibitor family. These inhibitors (by their properties) play a role in the feeding process of the tick. Development of antibodies against these proteins may be used to impair the normal feeding and consequently, the parasite would be no longer viable. (C) 1999 Elsevier Science B.V. All rights reserved. en
dc.format.extent 171-177
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Immunopharmacology
dc.rights Acesso restrito
dc.subject serine proteinase inhibitors en
dc.subject tick en
dc.subject blood coagulation en
dc.subject APTT en
dc.title A double headed serine proteinase inhibitor - human plasma kallikrein and elastase inhibitor - from Boophilus microplus larvae en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.description.affiliation UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliation EMBRAPA, CNPGC, C Grande, MS, Brazil
dc.description.affiliationUnifesp UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1016/S0162-3109(99)00074-0
dc.description.source Web of Science
dc.identifier.wos WOS:000084080100028


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