Effects of kinins upon cytosolic calcium concentrations in mouse mesangial cells

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dc.contributor.author Campos, A. H.
dc.contributor.author Calixto, J. B.
dc.contributor.author Schor, N.
dc.date.accessioned 2016-01-24T12:30:56Z
dc.date.available 2016-01-24T12:30:56Z
dc.date.issued 1999-12-01
dc.identifier http://dx.doi.org/10.1016/S0162-3109(99)00056-9
dc.identifier.citation Immunopharmacology. Amsterdam: Elsevier Science Bv., v. 45, n. 1-3, p. 39-49, 1999.
dc.identifier.issn 0162-3109
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26185
dc.description.abstract Bradykinin (BK) induces increases in cytosolic calcium concentration [Ca++](i) in several cell lines. Because the role of BK in the renal system, particularly in mesangial cell (MC), is not clear, we investigated the effects of kinins on [Ca++](i) in mouse-immortalized MC. [Ca++](i) was evaluated by spectrofluorometry and expressed as a ratio between the obtained and basal [Ca++](i). BK (0.1 mu M) induced a non-sustained increase in [Ca++](i) (4.70 +/- 0.27; N = 28). A similar effect was observed with the B-2 receptor agonist, Tyr(8)-BK (0.1 mu M, 3.34 +/- 0.48; N = 7), while B-1 receptor agonists, des-Arg(10)-Kal-lidin (Kal) (1 mu M, N = 11) and des-Arg(9)-BK (1 mu M, N = 8), exhibited only discrete responses (1.45 +/- 0.08 and 1.12 +/- 0.04, respectively). Cross-desensitization was seen between BK and Tyr(8)-BK, but not between BK and des-Arg(10)-Kal. the BK response was decreased (5.09 +/- 0.30, N = 6 to 1.57 +/- 0.12, N = 7, P < 0.001) by the B-2 receptor antagonist HOE 140 (0.1 mu M, 15 min), while the B-1 receptor antagonist des-Arg(9)-[Leu(8)]-BK (1 mu M, 15 min) had no effect on BK or des-Arg(10)-Kal actions. Incubation of cells with Escherichia coli lipopolysaccharide (100 mu g/ml, 24 h) alone or in association with tumor necrosis factor-alpha (TNF-alpha) (10 ng/ml, N = 6) did not enhance B-1 agonist responses. BK was inhibited by repeated cell washouts in zero Ca++ solution (2.04 +/- 0.19, N = 6 r < 0.001), and the residual response was almost abolished by thapsigargin (Thaps) a sarcoplasmic reticulum (SR) calcium-ATPase inhibitor (1 mu M) (1.18 +/- 0.08, N = 5 P < 0.001). Additionally, BK was not inhibited by verapamil (50 mu M), nifedipine (30 mu M), Ni++ (300 mu M) or La+++ (10 mu M). in conclusion, BK induces [Ca++](i) in mouse MC mainly by B-2 receptor activation. B-1 receptors have a minor role in this phenomenon even in the presence of known B1 receptor synthesis inducers. Finally, BK mobilizes extracellular calcium sources and, to a lesser extent, intracellular Thaps-sensitive calcium stores. the ion channels involved in calcium influx remain to be detected. (C) 1999 Elsevier Science B.W. All rights reserved. en
dc.format.extent 39-49
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Immunopharmacology
dc.rights Acesso restrito
dc.subject mesangial cell en
dc.subject bradykinin en
dc.subject cytosolic calcium en
dc.subject bradykinin receptors en
dc.title Effects of kinins upon cytosolic calcium concentrations in mouse mesangial cells en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal de Santa Catarina (UFSC)
dc.description.affiliation Universidade Federal de São Paulo, Dept Med, Div Nephrol, BR-04023900 São Paulo, Brazil
dc.description.affiliation Univ Fed Santa Catarina, Ctr Biol Sci, Div Pharmacol, Florianopolis, SC, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Med, Div Nephrol, BR-04023900 São Paulo, Brazil
dc.identifier.doi 10.1016/S0162-3109(99)00056-9
dc.description.source Web of Science
dc.identifier.wos WOS:000084080100008

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