Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron

Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron

Autor Schanstra, J. P. Google Scholar
Marin-Castano, M. E. Google Scholar
Alric, C. Google Scholar
Pesquero, J. B. Google Scholar
Claire, M. Google Scholar
Girolami, J. P. Google Scholar
Bascands, J. L. Google Scholar
Instituição CHU Rangueil
Universidade Federal de São Paulo (UNIFESP)
Resumo The kinin B1-receptor which is absent or expressed at very low levels under physiological conditions is strongly induced under inflammatory conditions. It has been shown that B1-receptor induction during inflammation involves interleukin-1 beta (IL-1 beta) production and activation of nuclear factor-kappa B (NF-kappa B). Since bradykinin (BK), the B2-receptor agonist induces IL-1 beta expression and activates NF-kappa B, we have analysed the effect of B2-receptor activation in cultured human lung fibroblasts cells on B1-receptor expression by a semiquantitative RT-PCR analysis. Treatment with BK resulted in a significant increase in the expression of B1-receptor mRNA which was abolished by a specific B2-receptor antagonist. This result suggests that B2-receptor activation can prime the expression of B1-receptors. Although the renal localisation of the B2-receptor has been thoroughly studied, nothing is known about the distribution of the B1-receptor in the kidney. Using a combination of microdissection and a semiquantitative, RT-PCR/Southern blot analysis we showed the absence of B1-receptors under physiological conditions in 10 microdissected rat nephron segments. However, 18 h LPS-treatment induced significant expression of the B1-receptor in all, but one segment. These studies provide the first molecular basis for the observed changes in renal haemodynamics after B1-agonist infusion in animal kidney models. (C) 1999 Elsevier Science B.V. All rights reserved.
Palavra-chave bradykinin B1-receptor
up-regulation
inflammation
nephron
microdissection
Idioma Inglês
Data de publicação 1999-12-01
Publicado em Immunopharmacology. Amsterdam: Elsevier B.V., v. 45, n. 1-3, p. 29-34, 1999.
ISSN 0162-3109 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 29-34
Fonte http://dx.doi.org/10.1016/S0162-3109(99)00054-5
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000084080100006
Endereço permanente http://repositorio.unifesp.br/handle/11600/26184

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