Physical mapping of a 670-kb region of chromosomes XVI and XVII from the human protozoan parasite Trypanosoma cruzi encompassing the genes for two immunodominant antigens

Physical mapping of a 670-kb region of chromosomes XVI and XVII from the human protozoan parasite Trypanosoma cruzi encompassing the genes for two immunodominant antigens

Autor Santos, Marcia Regina Machado Autor UNIFESP Google Scholar
Lorenzi, Hernan Google Scholar
Porcile, Patricio Autor UNIFESP Google Scholar
Carmo, Mirian Silva do Autor UNIFESP Google Scholar
Schijman, Alejandro Google Scholar
Brandao, Adeilton Google Scholar
Araya, Jorge Enrique Autor UNIFESP Google Scholar
Gomes, Henrique B M Autor UNIFESP Google Scholar
Chiurillo, Miguel A. Google Scholar
Ramirez, José L. Google Scholar
Degrave, Wim M. Google Scholar
Levin, Mariano J. Google Scholar
Silveira, José Franco da Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Invest Engenieria Genet & Biol Mol
Fiocruz
Cent Univ Venezuela
Resumo As part of the Trypanosoma cruzi Genome Initiative, we have mapped a large portion of the chromosomal bands XVI (2.3 Mb) and XVII (2.6 Mb) containing the highly repetitive and immunodominant antigenic gene families h49 and j18. Restriction mapping of the isolated chromosomal bands and hybridization with chromosome specific gene probes showed that genes h49 and j18 are located in a pair of size-polymorphic homologous chromosomes. To construct the integrated map of the chromosomes harboring the h49 and j18 loci, we used YAC, cosmid, slid lambda phage overlapping clones, and long range restriction analysis using a variety of probes (i.e., known gene sequences, ESTs, polymorphic repetitive sequences, anonymous sequences, STSs generated from the YAC ends). the total length covered by the YAC contig was approximately 670 kb, and its map agreed and was complementary to the one obtained by long-range restriction Fragment analysis. Average genetic marker spacing in a 105 kb region around h49 and j18 genes was estimated to be 6.2 kb/marker. We have detected some polymorphism in the H49/JL8 antigens-encoding chromosomes, affecting also the coding regions. the physical map of this region, together with the isolation of specific chromosome markers, will contribute in the global effort to sequence the nuclear genome of this parasite.
Idioma Inglês
Data de publicação 1999-12-01
Publicado em Genome Research. Plainview: Cold Spring Harbor Lab Press, v. 9, n. 12, p. 1268-1276, 1999.
ISSN 1088-9051 (Sherpa/Romeo, fator de impacto)
Publicador Cold Spring Harbor Lab Press
Extensão 1268-1276
Fonte http://dx.doi.org/10.1101/gr.9.12.1268
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000084550600013
Endereço permanente http://repositorio.unifesp.br/handle/11600/26181

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