Heterologous expression of a Trypanosoma cruzi surface glycoprotein (gp82) in mammalian cells indicates the existence of different signal sequence requirements and processing

Heterologous expression of a Trypanosoma cruzi surface glycoprotein (gp82) in mammalian cells indicates the existence of different signal sequence requirements and processing

Autor Ramirez, M. I. Google Scholar
Boscardin, S. B. Google Scholar
Han, S. W. Google Scholar
Paranhos-Baccala, G. Google Scholar
Yoshida, N. Google Scholar
Kelly, J. M. Google Scholar
Mortara, R. A. Google Scholar
Silveira, J. F. de Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Ecole Normale Super Lyon
London Sch Hyg & Trop Med
Resumo Metacyclic trypomastigotes of Trypanosoma cruzi express a developmentally regulated 82 kDa surface glycoprotein (gp82) that has been implicated in the mammalian cell invasion. When the non-infective epimastigote stage of the parasite was transfected with a vector containing the gp82 gene, an 82 kDa surface glycoprotein, which was indistinguishable from the metacyclic stage protein, was expressed. in contrast, when the same gene was expressed in transfected mammalian cells, although a large amount of protein was produced, it was not imported into the endoplasmic reticulum and glycosylated. This blockage in targeting and processing could be partially compensated for by the addition of a virus haemagglutinin signal peptide to the amino terminus of gp82. Thus, the requirements for membrane protein processing are distinct in mammals and T cruzi, and an intrinsic feature of the gp82 prevents subsequent sorting to the mammalian cell surface. These results could be useful in the development of new DNA vaccines against T. cruzi employing parasite genes encoding immunodominant surface glycoproteins.
Palavra-chave cellular trafficking
confocal microscopy
membrane protein processing
metacyclic trypomastigote
N-glycosylation
signal peptide
transgenic epimastigote
Vero cells
Idioma Inglês
Data de publicação 1999-11-01
Publicado em Journal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 46, n. 6, p. 557-565, 1999.
ISSN 1066-5234 (Sherpa/Romeo, fator de impacto)
Publicador Soc Protozoologists
Extensão 557-565
Fonte http://dx.doi.org/10.1111/j.1550-7408.1999.tb05131.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000083518300001
Endereço permanente http://repositorio.unifesp.br/handle/11600/26177

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