Myocardial expression of rat bradykinin receptors and two tissue kallikrein genes in experimental diabetes

Myocardial expression of rat bradykinin receptors and two tissue kallikrein genes in experimental diabetes

Autor Tschope, C. Google Scholar
Walther, T. Google Scholar
Yu, M. H. Google Scholar
Reinecke, A. Google Scholar
Koch, M. Google Scholar
Seligmann, C. Google Scholar
Heringer, S. B. Google Scholar
Pesquero, J. B. Google Scholar
Bader, M. Google Scholar
Schultheiss, H. P. Google Scholar
Unger, T. Google Scholar
Instituição Free Univ Berlin
Max Delbruck Ctr Mol Med
Univ Kiel
Universidade Federal de São Paulo (UNIFESP)
Resumo To characterize the role of the kallikrein-kinin system in diabetic cardiopathy, we studied the effect of streptozotocin (STZ) on the regulation of the myocardial bradykinin (BK) receptors, the B-1 and B-2 type, and two tissue kallikrein genes, rat kallikrein 1 (rKLK1) and rKLK7, in severely hyperglycemic rats. Experiments were performed in STZ-induced diabetic male Wistar rats (n = 7) and compared to controls (n = 7). After extraction of myocardial total RNA, specific oligonucleotides were used to generate reverse transcription PCR (RT-PCR) products from myocardial rKLK1 and rKLK7 mRNA. Southern blot analyses of these RT-PCR products were hybridized with appropriate gene-specific oligonucleotide probes. Myocardial B-1 and B-2 receptor expression were analyzed by RNase protection assays using specific probes from the coding region of the receptor genes. Twelve weeks after diabetes induction, the rats were normotensive and hyperglycemic and polyuric. We observed an impairment of the main myocardial kinin-forming enzymes, indicated by a reduction of the expression of both, rKLK1 and rKLK7. At this time the myocardial expression of the B-1 receptor was not detectable in either group. Thus, the B-1 receptor does not play a regulatory role in either the healthy or in STZ-diabetic heart. in contrast, the B-2-receptor expression was detectable but did not differ significantly in either group. the reduced synthesis of myocardial tissue KLK implies a reduced capacity to generate BK in diabetic rats. This reduction is not compensated by elevated BK receptor levels. We suggest that alterations of the KKS may contribute to myocardial dysfunction in diabetes mellitus. (C) 1999 Elsevier Science B.V. All rights reserved.
Palavra-chave kallikrein
streptozotocin
diabetic cardiopathy
B-1 receptor
B-2 receptor
Idioma Inglês
Data de publicação 1999-10-15
Publicado em Immunopharmacology. Amsterdam: Elsevier B.V., v. 44, n. 1-2, p. 35-42, 1999.
ISSN 0162-3109 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 35-42
Fonte http://dx.doi.org/10.1016/S0162-3109(99)00109-5
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000083920300007
Endereço permanente http://repositorio.unifesp.br/handle/11600/26161

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta