Potential definition of the time of death from autolytic myocardial cells: a morphometric study

Potential definition of the time of death from autolytic myocardial cells: a morphometric study

Autor Munoz, D. R. Google Scholar
Almeida, M. de Google Scholar
Lopes, E. A. Google Scholar
Iwamura, ESM Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Morphometric methods and transmission electron microscopy were used to quantify modifications occurring in the mitochondria of dog myocardium during the first four hours of autolysis. Myocardial fragments were obtained from the outer free wall of the left ventricle, during anesthesia (control-zero) and at 15, 45, 120, and 240 min after cardiac arrest, maintaining the heart in situ at 22 degrees C. During the 240 min of autolysis, the main parameters evaluated showed: (a) a decrease in the number of mitochondria from 0.31 to 0.12 per mu m(3) of cytoplasm. the decrease over the first 45 min reached 50% of the initial value; (b) an increase in mitochondrial volume, three times greater after the first 45 min (from 0.92 to 2.68 mu m(3)) and four times greater after 240 min (from 0.92 to 3.79 mu m(3)); (c) an increase in mitochondrial outer membrane surface area from 5.51 to 12.54 mu m(2); (d) an increase in the surface area of individual mitochondria inner membrane and cristae from 27.60 to 56.96 mu m(2). the progressive nature of the alterations and the difference in the numerically expressed values allow correlation with the time of somatic death. the authors emphasize the need for further studies in order to complement the present study. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
Assunto forensic science
Idioma Inglês
Data 1999-10-11
Publicado em Forensic Science International. Clare: Elsevier Sci Ireland Ltd, v. 104, n. 2-3, p. 81-89, 1999.
ISSN 0379-0738 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 81-89
Fonte http://dx.doi.org/10.1016/S0379-0738(99)00054-7
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000083257900001
URI http://repositorio.unifesp.br/handle/11600/26160

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