High occurrence of Shiga toxin-producing Escherichia coli (STEC) in healthy cattle in Rio de janeiro State, Brazil

High occurrence of Shiga toxin-producing Escherichia coli (STEC) in healthy cattle in Rio de janeiro State, Brazil

Autor Cerqueira, AMF Google Scholar
Guth, BEC Google Scholar
Joaquim, R. M. Google Scholar
Andrade, JRC Google Scholar
Instituição Universidade Federal Fluminense (UFF)
Universidade Federal de São Paulo (UNIFESP)
Universidade do Estado do Rio de Janeiro (UERJ)
Resumo In order to evaluate the prevalence of Shiga toxin-producing Escherichia coli (STEC) strains, 197 fecal samples of healthy cattle from 10 dairy farms, four beef farms and one slaughterhouse at Rio de Janeiro State, Brazil, were examined for Shiga toxin (Stx) gene sequences by polymerase chain reaction (PCR). for presumptive isolation of O157:H7 E. coli, the Cefixime-potassium tellurite-sorbitol MacConkey Agar (CT-SMAC) was used. A high occurrence (71%) of Stx was detected, and was more frequently found among dairy cattle (82% vs. 53% in beef cattle), in which no differences were observed regarding the age of the animals. Dot blot hybridization with stx1 and stx2 probes revealed that the predominant STEC type was one that had the genes for both stx1 and stx2 in dairy cattle and one that had only the stx1 gene for beef cattle. Three (1.5%) O157:H7 E. coli strains were isolated from one beef and two dairy animals by the use of CT-SMAC. To our knowledge, this is the first report of O157:H7 isolation in Brazil. A PCR-based STEC detection protocol led to the isolation of STEC in 12 of 16 randomly selected PCR-positive stool samples. A total of 15 STEC strains belonging to 11 serotypes were isolated, and most of them (60%) had both stx1 and stx2 gene sequences. Cytotoxicity assays with HeLa and Vero cells revealed that all strains except two of serotype O157:H7 expressed Stx. the data point to the high prevalence of STEC in our environment and suggest the need for good control strategies for the prevention of contamination of animal products. (C) 1999 Elsevier Science B.V. All rights reserved.
Assunto Shiga toxin Escherichia coli
polymerase chain reaction
Idioma Inglês
Data 1999-10-01
Publicado em Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 70, n. 1-2, p. 111-121, 1999.
ISSN 0378-1135 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 111-121
Fonte http://dx.doi.org/10.1016/S0378-1135(99)00123-6
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000083906800011
URI http://repositorio.unifesp.br/handle/11600/26151

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