Acute effects of inhalable particles on the frog palate mucociliary epithelium

Acute effects of inhalable particles on the frog palate mucociliary epithelium

Author Macchione, Mariangela Google Scholar
Oliveira, Alexandre P. Google Scholar
Gallafrio, Cristina T. Google Scholar
Muchão, Fabio B. Google Scholar
Obara, Marcos T. Google Scholar
Guimarães, Eliane T. Google Scholar
Artaxo, Paulo Google Scholar
King, Malcolm Google Scholar
Lorenzi-Filho, Geraldo Google Scholar
Junqueira, Virginia Berlanga Campos Autor UNIFESP Google Scholar
Saldiva, Paulo Hilário Nascimento Google Scholar
Institution Universidade de São Paulo (USP)
Univ Alberta
Universidade Federal de São Paulo (UNIFESP)
Abstract This work was designed to evaluate the toxicity of inhalable particles less than or equal to 10 pm in aerodynamic diameter (PM10) collected from the urban air in São Paulo, Brazil, to the mucociliary apparatus using the frog palate preparation. Seven groups of frog palates were immersed in different concentrations of PM10 diluted in Ringer's solution during 120 min: 0 (control, n = 31); 50 (n = 10); 100 (n = 9); 500 (n = 28); 1,000 (n = 10); 5,000 (n = 11); and 10,000 mu g/m(3) (n = 10). Mucociliary transport and transepithelial potential difference were determined at 0, 30, 60, and 120 min exposure. Additional groups (control and 500 pg/m(3)) were studied by means of morphometric analyses (quantification of the amount of intraepithelial and surface mucins), measurement of cilia beat frequency, and quantification of total glutathione. Mucociliary transport and transepithelial potential difference were significantly decreased at higher concentrations of PM10 (p = 0.03 and p = 0.02, respectively). Exposure to PM10 also elicited a significant decrease of total glutathione (p = 0.003) and depletion of neutral intraepithelial mucins (p = 0.0461). These results show that PM10 can promote significant alterations in ciliated epithelium in vitro.
Keywords air pollution
cilia beat frequency
glutathione
mucociliary transport
morphometric analyses
Language English
Date 1999-10-01
Published in Environmental Health Perspectives. Res Triangle Pk: Us Dept Health Human Sciences Public Health Science, v. 107, n. 10, p. 829-833, 1999.
ISSN 0091-6765 (Sherpa/Romeo, impact factor)
Publisher Us Dept Health Human Sciences Public Health Science
Extent 829-833
Origin http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566620/
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000083143700028
URI http://repositorio.unifesp.br/handle/11600/26145

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