HIV type 1 tat gene heteroduplex mobility assay as a tool to establish epidemiologic relationships among HIV type 1-infected individuals

HIV type 1 tat gene heteroduplex mobility assay as a tool to establish epidemiologic relationships among HIV type 1-infected individuals

Autor Diaz, R. S. Google Scholar
Oliveira, C. F. de Google Scholar
Pardini, R. Google Scholar
Operskalski, E. Google Scholar
Mayer, A. J. Google Scholar
Busch, M. P. Google Scholar
Instituição Blood Ctr Pacific
Universidade Federal de São Paulo (UNIFESP)
Univ So Calif
Univ Calif San Francisco
Resumo Molecular biology techniques are increasingly used to study the molecular epidemiology of infectious diseases. Most of these methods are expensive and labor-intensive, the human immunodeficiency virus (HIV) has substantial genomic variation, such that HIVs from different individuals are genetically diverse, although mutation rates differ for distinct regions of the genome, Most studies of HIV linkage and molecular evolution have focused on env or gag regions. We show that heteroduplex mobility analysis of the first exon of the HIV tat gene provides a simple, rapid, inexpensive, and reliable discriminatory tool for the molecular differentiation of shared versus distinct HIV-1 quasispecies when epidemiologic relations need to be defined. tat, as a relatively conserved region, appears to be a better region than the more variable env region to establish HIV-1 epidemiological linkages.
Idioma Inglês
Data de publicação 1999-09-01
Publicado em Aids Research and Human Retroviruses. Larchmont: Mary Ann Liebert Inc Publ, v. 15, n. 13, p. 1151-1156, 1999.
ISSN 0889-2229 (Sherpa/Romeo, fator de impacto)
Publicador Mary Ann Liebert Inc Publ
Extensão 1151-1156
Fonte http://dx.doi.org/10.1089/088922299310241
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000082292700002
Endereço permanente http://repositorio.unifesp.br/handle/11600/26141

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