Glycosaminoglycans in components of the rabbit eye: synthesis and characterization

Glycosaminoglycans in components of the rabbit eye: synthesis and characterization

Autor Goes, R. M. Google Scholar
Laicine, E. M. Google Scholar
Porcionatto, M. A. Google Scholar
Nader, H. B. Google Scholar
Haddad, A. Google Scholar
Instituição Universidade de São Paulo (USP)
UNESP
Universidade Federal de São Paulo (UNIFESP)
Resumo Purpose. To trace the eye components involved in proteoglycan synthesis and to characterize the sulfated glycosaminoglycans which are associated to these macromolecules.Methods. Sodium [S-35]-sulfate was injected intravitreally and the rabbits were killed at different time intervals after the injection. the glycosaminoglycans of choroid, ciliary body, cornea, iris, lens capsule, retina and sclera were extracted and processed for estimations of their specific activities, and for electrophoresis plus autoradiography with or without previous treatment with specific enzymes. in addition, methacrylate sections of the eyes were analysed by autoradiography.Results. the peak of specific activities of the glycosaminoglycans of all eye components occurred at 2 days after the intravitreal injection of [S-35]-sulfate. the autoradiography of the agarose gels revealed three types of glycosaminoglycans, namely, heparan-, chondroitin- and dermatan sulfate, only in the retina. the other eye components contained heparan sulfate and either chondroitin or dermatan sulfate. Tissue autoradiography together with the biochemical techniques contributed to unravel the origin of the glycosaminoglycans in the eye components.Conclusions. the results of the present investigation have shown that heparan sulfate, contrasting to chondroitin sulfate and dermatan sulfate, is synthesized in all eye components studied and that the glycosaminoglycan composition differs according to the tissue of origin.
Palavra-chave glycosaminoglycans
eye
heparan sulfate
condroitin sulfate
dermatan sulfate
rabbit
rate of synthesis
Idioma Inglês
Data de publicação 1999-08-01
Publicado em Current Eye Research. Lisse: Swets Zeitlinger Publishers, v. 19, n. 2, p. 146-153, 1999.
ISSN 0271-3683 (Sherpa/Romeo, fator de impacto)
Publicador Swets Zeitlinger Publishers
Extensão 146-153
Fonte http://dx.doi.org/10.1076/ceyr.19.2.146.5324
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000082004600008
Endereço permanente http://repositorio.unifesp.br/handle/11600/26127

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