Cockroach allergens and asthma in Brazil: Identification of tropomyosin as a major allergen with potential cross-reactivity with mite and shrimp allergens

Cockroach allergens and asthma in Brazil: Identification of tropomyosin as a major allergen with potential cross-reactivity with mite and shrimp allergens

Author Santos, ABR Google Scholar
Chapman, M. D. Google Scholar
Aalberse, R. C. Google Scholar
Vailes, L. D. Google Scholar
Ferriani, VPL Google Scholar
Oliver, C. Google Scholar
Rizzo, M. C. Google Scholar
Naspitz, C. K. Google Scholar
Arruda, L. K. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Netherlands Red Cross
Univ Virginia
Abstract Background: Cockroaches produce several proteins that induce IgE antibody responses. Although cockroaches are abundant in warm and humid areas, sensitization to cockroach allergens has not been investigated in Brazil.Objective: the aims of this study were to investigate the frequency of cockroach allergy among patients with asthma, rhinitis, or both in Brazil and to identify American cockroach allergens.Methods: Skin tests using cockroach extracts were performed on children and young adults with asthma, rhinitis, or both. A Periplaneta americana complementary (c)DNA library was screened by using IgE antibodies from Brazilian patients allergic to cockroaches. Reactivity of an mAb directed to Dermatophagoides pteronyssinus tropomyosin against cockroach tissue was examined by immunofluorescence.Results: Cockroach allergy was present in 55% and 79% of the patients, as determined by using skin prick tests alone or combined prick and intradermal tests, respectively. Five cDNA clones reacted with IgE antibody and contained the same sequence. A representative clone (1300 bp), pa12, coded for a protein that reacted with 50% of the sera from patients allergic to cockroaches on plaque immunoassay and showed a high degree of homology to tropomyosins, particularly those from invertebrates. P americana tropomyosin showed 80%, 81%, and 82% sequence identity to tropomyosins from D pteronyssinus, D farinae, and shrimp, respectively, which have been previously defined as important allergens. An mAb directed against D pteronyssinus tropomyosin, which also recognizes shrimp tropomyosin, showed binding to cockroach striated muscle.Conclusion: Our results support the recommendation that cockroach extracts should be routinely used for the evaluation of patients with asthma, rhinitis, or both in Brazil. the identification of P americana tropomyosin as an important allergen Hill make it possible to investigate cross-reactivity among cockroaches, mites, and food derived from invertebrates.
Keywords cockroach
tropomyosin
asthma
mites
shrimp
allergy
Periplaneta americana
Language English
Date 1999-08-01
Published in Journal of Allergy and Clinical Immunology. St Louis: Mosby-year Book Inc, v. 104, n. 2, p. 329-337, 1999.
ISSN 0091-6749 (Sherpa/Romeo, impact factor)
Publisher Mosby-year Book Inc
Extent 329-337
Origin http://dx.doi.org/10.1016/S0091-6749(99)70375-1
Access rights Closed access
Type Article
Web of Science ID WOS:000082364800024
URI http://repositorio.unifesp.br/handle/11600/26125

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