Calomys callosus (Rodentia : Cricetidae) trophoblast cells as host cells to Toxoplasma gondii in early pregnancy

Calomys callosus (Rodentia : Cricetidae) trophoblast cells as host cells to Toxoplasma gondii in early pregnancy

Autor Ferro, EAV Google Scholar
Bevilacqua, E. Google Scholar
Favoreto, S. Google Scholar
Silva, DAO Google Scholar
Mortara, R. A. Google Scholar
Mineo, JR Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de Uberlândia (UFU)
Universidade Federal de São Paulo (UNIFESP)
Resumo The potential of the RH strain of Toxoplasma gondii to invade trophoblast. cells of the cricetid rodent Calomys callosus in a congenital infection in the initial third of pregnancy was investigated in this study using morphological and immunocytochemical approaches. the animals were intraperitoneally inoculated on the Ist day of pregnancy and the infection was observed on day 7. Various numbers of parasites could be observed inside the parasitophorous vacuoles in trophoblastic cells under light and electron microscopy. the trophoblast cells showed characteristics of healthy cells, and no alteration other than parasite vacuoles in their cytoplasm could be detected. Polyclonal or monoclonal anti-T. gondii antibodies (respectively, anti-T. gondii components and the major surface parasite antigen p30) labeled both the parasite surface and parasitophorous vacuole membranes, regardless of the number of parasites inside the compartment. in addition, p30-containing trails were detected in the extracellular matrix surrounding trophoblastic cells similar to those found with other parasites during locomotion and the invasion process. Our results show the ability of T. gondii to infect trophoblast cells during the early blastocyst-endometrial relationship and open new possibilities for more accurate study of the invasion process of this parasite and the role of the trophoblast as an embryo defense barrier.
Idioma Inglês
Data 1999-08-01
Publicado em Parasitology Research. New York: Springer Verlag, v. 85, n. 8-9, p. 647-654, 1999.
ISSN 0044-3255 (Sherpa/Romeo, fator de impacto)
Editor Springer
Extensão 647-654
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000081302800005

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