Leishmania: Overexpression and comparative structural analysis of the stage-regulated meta 1 gene

Leishmania: Overexpression and comparative structural analysis of the stage-regulated meta 1 gene

Autor Uliana, SRB Google Scholar
Goyal, N. Google Scholar
Freymuller, E. Google Scholar
Smith, D. F. Google Scholar
Instituição Universidade de São Paulo (USP)
Univ London Imperial Coll Sci Technol & Med
Universidade Federal de São Paulo (UNIFESP)
Cent Drug Res Inst
Resumo The meta I gene of Leishmania major is upregulated in metacyclic promastigotes and encodes an 11.5-kDa protein with no significant similarities to other proteins in the existing databases. in this paper, we characterize the homologous meta 1 genes in L. amazonensis and L. donovani. Proteins encoded by this gene in all three species present a high degree of identity. the meta 1 gene cannot be replaced by gene targeting in L. major, suggesting an essential role for the protein, at least in promastigotes. Overexpression of the meta 1 protein in L. amazonensis generates parasites that are more virulent than wild-type organisms in vivo. (C) 1999 Academic Press.
Palavra-chave Leishmania
differential gene expression
mutants
overexpression
virulence
Idioma Inglês
Data de publicação 1999-07-01
Publicado em Experimental Parasitology. San Diego: Academic Press Inc, v. 92, n. 3, p. 183-191, 1999.
ISSN 0014-4894 (Sherpa/Romeo, fator de impacto)
Publicador Academic Press Inc
Extensão 183-191
Fonte http://dx.doi.org/10.1006/expr.1999.4410
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000081427100003
Endereço permanente http://repositorio.unifesp.br/handle/11600/26101

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