A novel phospholipase A2, BJ-PLA2, from the venom of the snake Bothrops jararaca: Purification, primary structure analysis, and its characterization as a platelet-aggregation-inhibiting factor

A novel phospholipase A2, BJ-PLA2, from the venom of the snake Bothrops jararaca: Purification, primary structure analysis, and its characterization as a platelet-aggregation-inhibiting factor

Autor Serrano, SMT Google Scholar
Reichl, A. P. Google Scholar
Mentele, R. Google Scholar
Auerswald, E. A. Google Scholar
Santoro, M. L. Google Scholar
Sampaio, CAM Google Scholar
Camargo, ACM Google Scholar
Assakura, M. T. Google Scholar
Instituição Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Univ Munich
Resumo This paper describes the isolation and primary structure analysis of a new phospholipase A2 with platelet-aggregation-inhibiting activity from the venom of Bothrops jararaca. the protein, named BJ-PLA2, was isolated by means of ammonium sulfate precipitation and anion-exchange and reversed-phase chromatographies and behaved as a homogeneous single-chain protein on SDS-PAGE. Its amino acid sequence was determined by N-terminal sequencing and analysis of overlapped chemical and proteolytic fragments by automated Edman degradation and mass spectometry determination. BJ-PLA2 consists of 124 amino acid residues and has the structural features of snake venom class II phospholipases A2, Chemical modification with p-bromophenacylbromide caused complete loss of enzymatic activity and partially affected the platelet-aggregation-inhibiting activity of BJ-PLA2. (C) 1999 Academic Press.
Palavra-chave phospholipase A2
Bothrops jararaca venom
platelet aggregation
primary structure analysis
Idioma Inglês
Data de publicação 1999-07-01
Publicado em Archives of Biochemistry and Biophysics. San Diego: Academic Press Inc, v. 367, n. 1, p. 26-32, 1999.
ISSN 0003-9861 (Sherpa/Romeo, fator de impacto)
Publicador Academic Press Inc
Extensão 26-32
Fonte http://dx.doi.org/10.1006/abbi.1999.1230
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000081316800004
Endereço permanente http://repositorio.unifesp.br/handle/11600/26100

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