Identification of the telomere in Trypanosoma cruzi reveals highly heterogeneous telomere lengths in different parasite strains

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dc.contributor.author Freitas-Junior, Lúcio H. G [UNIFESP]
dc.contributor.author Marques-Porto, Rafael [UNIFESP]
dc.contributor.author Pirrit, Lindsay Ann
dc.contributor.author Schenkman, Sergio [UNIFESP]
dc.contributor.author Scherf, Artur
dc.date.accessioned 2016-01-24T12:30:50Z
dc.date.available 2016-01-24T12:30:50Z
dc.date.issued 1999-06-15
dc.identifier http://dx.doi.org/10.1093/nar/27.12.2451
dc.identifier.citation Nucleic Acids Research. Oxford: Oxford Univ Press, v. 27, n. 12, p. 2451-2456, 1999.
dc.identifier.issn 0305-1048
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26094
dc.description.abstract Here we describe the cloning and characterisation of the Trypanosoma cruzi telomere, in the Y strain, it is formed by typical GGGTTA repeats with a mean size of similar to 500 bp. Adjacent to the telomere repeats we found a DNA sequence with significant homology to the T.cruzi 85 kDa surface antigen (gp85), Examination of the telomere in nine T.cruzi strains reveals differences in the organisation of chromosome ends, in one group of strains the size of the telomere repeat is relatively homogeneous and short (0.5-1.5 kb) as in the Y strain, white in the other, the length of the repeat is very heterogeneous and significantly longer, ranging in size from 1 to >10 kb, These different strains can be grouped similarly to previously existing classifications based on isoenzyme loci. rRNA genes, mini-exon gene sequences, randomly amplified polymorphic DNA and rRNA promoter sequences, suggesting that differential control of telomere length and organisation appeared as an early event in T.cruzi evolution, Two-dimensional pulsed field gel electrophoresis analysis shows that some chromosomes carry telomeres which are significantly larger than the mean telomere length. Importantly, the T.cruzi telomeres are organised in nucleosomal and non-nucleosomal chromatin. en
dc.format.extent 2451-2456
dc.language.iso eng
dc.publisher Oxford Univ Press
dc.relation.ispartof Nucleic Acids Research
dc.rights Acesso aberto
dc.title Identification of the telomere in Trypanosoma cruzi reveals highly heterogeneous telomere lengths in different parasite strains en
dc.type Artigo
dc.rights.license http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.contributor.institution Inst Pasteur
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Inst Pasteur, CNRS URA 1960, Unite Biol Interact Hote Parasite, F-75724 Paris 15, France
dc.description.affiliation Univ São Paulo, Dept Parasitol, Inst Ciencias Biomed 2, ICB 2, BR-05508900 São Paulo, Brazil
dc.description.affiliation UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1093/nar/27.12.2451
dc.description.source Web of Science
dc.identifier.wos WOS:000080969500002



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