Trypanosoma rangeli: Discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene

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dc.contributor.author Souto, R. P.
dc.contributor.author Vargas, N.
dc.contributor.author Zingales, B.
dc.date.accessioned 2016-01-24T12:30:47Z
dc.date.available 2016-01-24T12:30:47Z
dc.date.issued 1999-04-01
dc.identifier http://dx.doi.org/10.1006/expr.1998.4380
dc.identifier.citation Experimental Parasitology. San Diego: Academic Press Inc, v. 91, n. 4, p. 306-314, 1999.
dc.identifier.issn 0014-4894
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26054
dc.description.abstract Three synthetic oligonucleotides corresponding to sequences within the D7a divergent domain of the large subunit ribosomal RNA gene have been used to amplify the total DNA of Trypanosoma rangeli and Trypanosoma cruzi, two morphologically similar protozoa with overlapping geographical distribution and hosts. the two organisms may be distinguished by the electrophoretic mobilities of their respective amplification products. for T. rangeli a 210-bp product was obtained. the presence of this fragment was confirmed in 14 T. rangeli strains. for T. cruzi two possible amplification products were originated: a 265-bp DNA fragment for strains typed as lineage 1 and a 250-bp fragment for lineage 2 strains. Eleven unidentified trypanosome stocks, recently isolated from Amazonian vectors, could be discriminated using the proposed assay. the potential field application of multiplex PCR was further demonstrated by identification of the two parasite species in samples containing intestinal tract and feces of triatomines. in the present study we have also amplified the D7a domain of several trypanosomatids employing primers complementary to the conserved flanking regions. Size and sequence polymorphisms were observed, indicating that this region could also be explored as a target for specific detection of other members of the Trypanosomatidae family. (C) 1999 Academic Press. en
dc.format.extent 306-314
dc.language.iso eng
dc.publisher Academic Press Inc
dc.relation.ispartof Experimental Parasitology
dc.rights Acesso restrito
dc.subject Trypanosoma rangeli en
dc.subject Trypanosoma cruzi en
dc.subject Trypanosoma brucei en
dc.subject Trypanoplasma borreli en
dc.subject Leishmania (V.) braziliensis en
dc.subject Leishmania (L.) chagasi en
dc.subject Leishmania (L.) amazonensis en
dc.subject Crithidia fasciculata en
dc.subject Rhodnius brethesi en
dc.subject Rhodnius prolixus en
dc.subject trypanosomatid en
dc.subject ribosomal RNA genes en
dc.subject divergent domains en
dc.subject PCR en
dc.subject detection en
dc.subject epidemiology en
dc.title Trypanosoma rangeli: Discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Inst Quim, Dept Bioquim, BR-05599970 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Inst Quim, Dept Bioquim, BR-05599970 São Paulo, Brazil
dc.identifier.doi 10.1006/expr.1998.4380
dc.description.source Web of Science
dc.identifier.wos WOS:000079541800003



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