Trypanosoma rangeli: Discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene

Trypanosoma rangeli: Discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene

Author Souto, R. P. Google Scholar
Vargas, N. Google Scholar
Zingales, B. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Three synthetic oligonucleotides corresponding to sequences within the D7a divergent domain of the large subunit ribosomal RNA gene have been used to amplify the total DNA of Trypanosoma rangeli and Trypanosoma cruzi, two morphologically similar protozoa with overlapping geographical distribution and hosts. the two organisms may be distinguished by the electrophoretic mobilities of their respective amplification products. for T. rangeli a 210-bp product was obtained. the presence of this fragment was confirmed in 14 T. rangeli strains. for T. cruzi two possible amplification products were originated: a 265-bp DNA fragment for strains typed as lineage 1 and a 250-bp fragment for lineage 2 strains. Eleven unidentified trypanosome stocks, recently isolated from Amazonian vectors, could be discriminated using the proposed assay. the potential field application of multiplex PCR was further demonstrated by identification of the two parasite species in samples containing intestinal tract and feces of triatomines. in the present study we have also amplified the D7a domain of several trypanosomatids employing primers complementary to the conserved flanking regions. Size and sequence polymorphisms were observed, indicating that this region could also be explored as a target for specific detection of other members of the Trypanosomatidae family. (C) 1999 Academic Press.
Keywords Trypanosoma rangeli
Trypanosoma cruzi
Trypanosoma brucei
Trypanoplasma borreli
Leishmania (V.) braziliensis
Leishmania (L.) chagasi
Leishmania (L.) amazonensis
Crithidia fasciculata
Rhodnius brethesi
Rhodnius prolixus
trypanosomatid
ribosomal RNA genes
divergent domains
PCR
detection
epidemiology
Language English
Date 1999-04-01
Published in Experimental Parasitology. San Diego: Academic Press Inc, v. 91, n. 4, p. 306-314, 1999.
ISSN 0014-4894 (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc
Extent 306-314
Origin http://dx.doi.org/10.1006/expr.1998.4380
Access rights Closed access
Type Article
Web of Science ID WOS:000079541800003
URI http://repositorio.unifesp.br/handle/11600/26054

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