Retinal pigment epithelial cells are heterogeneous in their expression of MHC-II after stimulation with interferon-gamma

Retinal pigment epithelial cells are heterogeneous in their expression of MHC-II after stimulation with interferon-gamma

Author Casella, AMB Google Scholar
Taba, K. E. Google Scholar
Kimura, H. Google Scholar
Spee, C. Google Scholar
Cardillo, J. A. Google Scholar
Ryan, S. J. Google Scholar
Hinton, D. R. Google Scholar
Institution Univ So Calif
Universidade Estadual de Londrina (UEL)
Universidade Federal de São Paulo (UNIFESP)
Abstract The pattern of interferon-gamma-induced major histocompatibility complex Class II antigen expression was evaluated on the retinal pigment epithelium. Experiments were performed in vitro using explant cultures of aged and fetal human eyes and in vivo in albino rabbits. the human explants were stimulated with 50 U ml(-1) interferon-gamma for 3 days prior to immunostaining for Class II. the rabbit eyes were subretinally injected in vivo with 50 mu l of interferon-gamma (500 U ml(-1)) and analyzed immunohistochemically 3 days later. A heterogeneous pattern of Class LI expression was present in the interferon-gamma-stimulated retinal pigment epithelial cells, in both the in vivo and the in vitro experiments. in aged human eyes the percent of Class-II positive cells was higher in the periphery than in the posterior pole (macular region) after interferon-gamma stimulation (P < 0.01). No such difference was found in the fetal eyes. These data demonstrate that retinal pigment epithelial cells are heterogeneous in their response to interferon-gamma. the results are supportive of previous studies demonstrating the structural and proliferative heterogeneity of the retinal pigment epithelium. Together, these studies provide support for the possibility of functional retinal pigment epithelial heterogeneity. (C) 1999 Academic Press.
Keywords RPE
heterogeneity
MHC class II
aging
PVR
Language English
Date 1999-04-01
Published in Experimental Eye Research. London: Academic Press Ltd, v. 68, n. 4, p. 423-430, 1999.
ISSN 0014-4835 (Sherpa/Romeo, impact factor)
Publisher Academic Press Ltd
Extent 423-430
Origin http://dx.doi.org/10.1006/exer.1998.0621
Access rights Closed access
Type Article
Web of Science ID WOS:000079745000004
URI http://repositorio.unifesp.br/handle/11600/26053

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