Crystallization and preliminary X-ray data for the human transthyretin-retinol-binding protein (RBP) complex bound to an anti-REP Fab

Crystallization and preliminary X-ray data for the human transthyretin-retinol-binding protein (RBP) complex bound to an anti-REP Fab

Autor Malpeli, G. Google Scholar
Zanotti, G. Google Scholar
Gliubich, F. Google Scholar
Rizzotto, A. Google Scholar
Nishida, S. K. Google Scholar
Folli, C. Google Scholar
Berni, R. Google Scholar
Instituição Univ Parma
Univ Padua
CNR
Univ Milan
Universidade Federal de São Paulo (UNIFESP)
Resumo A macromolecular complex of human transthyretin, human retinol-binding protein and an anti-retinol-binding-protein Fab was crystallized by vapour diffusion in sitting drops. Diffraction from these crystals at cryogenic temperatures was consistent with the space group C222, with cell parameters a = 159.34, b = 222.40 and c = 121.27 Angstrom. Crystals diffracted to a resolution limit of 3.36 Angstrom using synchrotron radiation. Based on a 2:2:1 stoichiometry for the Fab-retinol-binding-protein-transthyretin complex and the presence of one such complex per asymmetric unit, a reasonable V-m coefficient of 2.74 Angstrom(3) Da(-1) could be estimated.
Idioma Inglês
Data de publicação 1999-01-01
Publicado em Acta Crystallographica Section D-biological Crystallography. Copenhagen: Munksgaard Int Publ Ltd, v. 55, p. 276-278, 1999.
ISSN 0907-4449 (Sherpa/Romeo, fator de impacto)
Publicador Munksgaard Int Publ Ltd
Extensão 276-278
Fonte http://dx.doi.org/10.1107/S0907444998007860
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000078314000038
Endereço permanente http://repositorio.unifesp.br/handle/11600/26021

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