Involvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophages

Involvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophages

Autor Almeida, SR Google Scholar
Unterkircher, C. S. Google Scholar
Camargo, Z. P. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
UNESP
Resumo The yeast form of Paracoccidioides brasiliensis, the causative agent of a deep mycosis in humans, is known to be phagocytized by, and to multiply inside, macrophages. in this work we describe the involvement of gp43, a major antigenic protein of P. brasiliensis, in the initial steps of attachment of the fungus to macrophages. Anti-gp43 F(ab) polyclonal fragments were capable of inhibiting phagocytosis in a concentration-dependent manner. Sheep red blood cells sensitized with purified gp43 were more endocytized than SF:BC alone, and this process was also inhibited by anti-gp43 F(ab) fragments. Inhibition tests indicated the involvement of fucose and mannose residues in the phagocytosis of the fungus and of SRBC-gp43 by macrophages. Taken together, these results suggest that gp43 may be involved in the adherence and uptake of the fungus by murine peritoneal macrophages, and that this binding may be dependent on monosaccharide residues that are part of the gp43 glycoprotein.
Palavra-chave macrophages
Paracoccidioides brasiliensis
paracoccidioidomycosis
phagocytosis
Idioma Inglês
Data de publicação 1998-12-01
Publicado em Medical Mycology. Oxford: Blackwell Science Ltd, v. 36, n. 6, p. 405-411, 1998.
ISSN 1369-3786 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Science Ltd
Extensão 405-411
Fonte http://dx.doi.org/10.1080/02681219880000641
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000077858400008
Endereço permanente http://repositorio.unifesp.br/handle/11600/25993

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