Purification and partial characterization of kininogenase activity from Schistosoma mansoni adult worms

Purification and partial characterization of kininogenase activity from Schistosoma mansoni adult worms

Autor Carvalho, W. S. Google Scholar
Lopes, C. T. Google Scholar
Juliano, L. Google Scholar
Coelho, PMZ Google Scholar
Cunha-Melo, JR Google Scholar
Beraldo, W. T. Google Scholar
Pesquero, J. L. Google Scholar
Instituição Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
Resumo An enzyme presenting kallikrein-like activity (designated sK1) was purified from the supernatant of Schistosoma mansoni adult worm homogenate. the enzyme cleaves bradykinin from purified rat plasma kininogen. Activity was optimal at pH 9.0 and the enzyme showed amidolytic activity, since it hydrolysed the kallikrein synthetic substrate D-Pro-Phe-Arg-p-nitroanilide. the activity of sK1 upon rat plasma kininogen was strongly inhibited by the serine proteinase inhibitors phenylmethanesulfonyl fluoride, aprotinin or soybean trypsin inhibitor, but not by ethylenediaminetetraacetic acid or sodium tetrathionate. the molecular mass of sK1, as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, was 66 kDa and the pI value, estimated by analytical chromatofocusing, was 4.2. Physical and chemical properties suggest that sK1 is a serine proteinase of the kallikrein family. Evidence is presented which suggests that sK1 is a component of the tegumental surface of the parasite and the levels of its activity in the male adult worm are approximately 21 times higher than those in the female adult worm. the intravenous injection of 3 mu g of sK1 into an anaesthetized rat induced a drastic reduction in the arterial blood pressure of the animal. This effect lasted for about 1 min, and was followed by a progressive recovery of the arterial pressure. Neither bradycardia nor cardiac arrhythmias were noticed, suggesting a peripheral vasodilation effect. the presence of sK1 on the surface of adult male worms could play an important role in the wandering capacity of coupled worms into the visceral vasculature of the host.
Palavra-chave kallikrein (EC 3.4.21.35)
kinin
kininogenase
Schistosoma mansoni
serine proteinase
vasodilation
wandering capacity
Idioma Inglês
Data de publicação 1998-10-01
Publicado em Parasitology. New York: Cambridge Univ Press, v. 117, p. 311-319, 1998.
ISSN 0031-1820 (Sherpa/Romeo, fator de impacto)
Publicador Cambridge Univ Press
Extensão 311-319
Fonte http://dx.doi.org/10.1017/S0031182098003175
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000077027900003
Endereço permanente http://repositorio.unifesp.br/handle/11600/25966

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