Stimulation of heparan sulfate proteoglycan synthesis and secretion during G(1) phase induced by growth factors and PMA

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dc.contributor.author Porcionatto, M. A.
dc.contributor.author Moreira, C. R.
dc.contributor.author Lotfi, CFP
dc.contributor.author Armelin, H. A.
dc.contributor.author Dietrich, C. P.
dc.contributor.author Nader, H. B.
dc.date.accessioned 2016-01-24T12:30:39Z
dc.date.available 2016-01-24T12:30:39Z
dc.date.issued 1998-09-15
dc.identifier http://dx.doi.org/10.1002/(SICI)1097-4644(19980915)70:4<563
dc.identifier.citation Journal of Cellular Biochemistry. New York: Wiley-liss, v. 70, n. 4, p. 563-572, 1998.
dc.identifier.issn 0730-2312
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/25958
dc.description.abstract Fetal calf serum (FCS) and PMA(phorbol 12-myristate-13-acetate) specifically stimulate the synthesis of heparan sulfate proteoglycan in endothelial cells. Staurosporine and n-butanol, kinase inhibitors, abolish the PMA effect. Forskolin and 8-bromo adenosine 3':5'-cyclic monophosphate, activators of, respectively, adenylate cyclase and protein kinase A cannot reproduce the PMA effect. the kinetics of cell entry into S phase of the endothelial cells was determined by DNA synthesis ([H-3]thymidine and Br-dU incorporation), and flow cytometry. the mitogenic effect of fetal calf serum is abolished by PMA. Also, PMA pre-treatment inhibits the enhanced synthesis of heparan sulfate proteoglycan after a second PMA exposure. Remarkably, the stimulation of heparan sulfate proteoglycan synthesis by fetal calf serum and PMA seems to be mainly restricted to G(1) phase. Therefore fetal calf serum and PMA cause an enhanced synthesis of heparan sulfate proteoglycan, and PMA causes a cell cycle block at G(1) phase. J. Cell. Biochem. 70:563-572, 1998. (C) 1998 Wiley-Liss, Inc. en
dc.format.extent 563-572
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Journal of Cellular Biochemistry
dc.rights Acesso restrito
dc.subject heparan sulfate and growth factors en
dc.subject heparan sulfate and phorbol ester en
dc.subject heparan sulfate and cell cycle en
dc.subject proteoglycans and cell cycle en
dc.subject cell cycle en
dc.subject phorbol ester and heparan sulfate en
dc.subject heparan sulfate and PKC en
dc.title Stimulation of heparan sulfate proteoglycan synthesis and secretion during G(1) phase induced by growth factors and PMA en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Quim, Dept Bioquim, BR-05508 São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1002/(SICI)1097-4644(19980915)70:4<563
dc.description.source Web of Science
dc.identifier.wos WOS:000075178200012



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