Stimulation of heparan sulfate proteoglycan synthesis and secretion during G(1) phase induced by growth factors and PMA

Stimulation of heparan sulfate proteoglycan synthesis and secretion during G(1) phase induced by growth factors and PMA

Autor Porcionatto, M. A. Google Scholar
Moreira, C. R. Google Scholar
Lotfi, CFP Google Scholar
Armelin, H. A. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo Fetal calf serum (FCS) and PMA(phorbol 12-myristate-13-acetate) specifically stimulate the synthesis of heparan sulfate proteoglycan in endothelial cells. Staurosporine and n-butanol, kinase inhibitors, abolish the PMA effect. Forskolin and 8-bromo adenosine 3':5'-cyclic monophosphate, activators of, respectively, adenylate cyclase and protein kinase A cannot reproduce the PMA effect. the kinetics of cell entry into S phase of the endothelial cells was determined by DNA synthesis ([H-3]thymidine and Br-dU incorporation), and flow cytometry. the mitogenic effect of fetal calf serum is abolished by PMA. Also, PMA pre-treatment inhibits the enhanced synthesis of heparan sulfate proteoglycan after a second PMA exposure. Remarkably, the stimulation of heparan sulfate proteoglycan synthesis by fetal calf serum and PMA seems to be mainly restricted to G(1) phase. Therefore fetal calf serum and PMA cause an enhanced synthesis of heparan sulfate proteoglycan, and PMA causes a cell cycle block at G(1) phase. J. Cell. Biochem. 70:563-572, 1998. (C) 1998 Wiley-Liss, Inc.
Palavra-chave heparan sulfate and growth factors
heparan sulfate and phorbol ester
heparan sulfate and cell cycle
proteoglycans and cell cycle
cell cycle
phorbol ester and heparan sulfate
heparan sulfate and PKC
Idioma Inglês
Data de publicação 1998-09-15
Publicado em Journal of Cellular Biochemistry. New York: Wiley-liss, v. 70, n. 4, p. 563-572, 1998.
ISSN 0730-2312 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 563-572
Fonte http://dx.doi.org/10.1002/(SICI)1097-4644(19980915)70:4<563
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000075178200012
Endereço permanente http://repositorio.unifesp.br/handle/11600/25958

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