Trypanosoma cruzi: Effect of protein kinase inhibitors and cytoskeletal protein organization and expression on host cell invasion by amastigotes and metacyclic trypomastigotes

Trypanosoma cruzi: Effect of protein kinase inhibitors and cytoskeletal protein organization and expression on host cell invasion by amastigotes and metacyclic trypomastigotes

Autor Procopio, D. O. Google Scholar
Silva, S. da Google Scholar
Cunningham, C. C. Google Scholar
Mortara, R. A. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Although trypomastigotes are regarded as the classic infective forms of T. cruzi, amastigotes generated extracellularly or released from infected cells during lysis may circulate and infect other cells. We have compared the infectivity of metacyclic trypomastigotes and extracellular amastigotes toward HeLa and Vero cells and observed that amastigotes were capable of invading both HeLa and Vero cells to a much higher degree than the corresponding metacyclic forms. Second, cell microfilament or microtubule disruption inhibited amastigote but not trypomastigote entry. Third, cells with altered expression in cytoskeletal components (ABP or gelsolin) internalize amastigotes and trypomastigotes with highly contrasting fashion. Fourth, protein kinase inhibitors such as genistein and staurosporine affect the internalization of amastigotes and trypomastigotes in a host-cell-dependent manner. Our results suggest that extracellular amastigotes and metacyclic trypomastigotes utilize mechanisms to invade host cells with particular features for each I: cruzi form and for each host cell. When internalized, both forms associate to lysosomes of HeLa cells. (C) 1998 Academic Press.
Palavra-chave Trypanosoma cruzi
trypomastigotes
amastigotes
host cell cytoskeleton
protein phosphorylation inhibitors
cellular invasion
Idioma Inglês
Data de publicação 1998-09-01
Publicado em Experimental Parasitology. San Diego: Academic Press Inc, v. 90, n. 1, p. 1-13, 1998.
ISSN 0014-4894 (Sherpa/Romeo, fator de impacto)
Publicador Academic Press Inc
Extensão 1-13
Fonte http://dx.doi.org/10.1006/expr.1998.4314
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000075423100001
Endereço permanente http://repositorio.unifesp.br/handle/11600/25945

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