Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein

Mapping of B cell epitopes in an immunodominant antigen of Trypanosoma cruzi using fusions to the Escherichia coli LamB protein

Author Pereira, Catia Mendes Autor UNIFESP Google Scholar
Yamauchi, Lucy M. Google Scholar
Levin, Mariano J. Google Scholar
Silveira, José Franco da Google Scholar
Castilho, Beatriz A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Ingebi
Abstract The JL8 protein antigen from Trypanosoma cii , a dominant immunogen in man, has been characterized as containing tandem amino acid repeats. Here, we describe the use of the LamB protein of Escherichia coli as a carl icr of JL8 derived sequences in order to map the immunodominant B cell epitopes in this antigen. Five different sequences of JL8 were inserted in the LamB protein and the JL8-LamB fusion proteins were tested by ELISA with human chronic chagasic sera. the fusion carrying the sequence AEKQKAAEATKVAE was recognized by most sera. This protein was also capable of inhibiting the binding of human chagasic antibodies to GST-JL8 in competitive ELISA suggesting that it contains an immunodominant B cell epitope of JL8. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Keywords Trypanosoma cruzi
immunodominant repetitive antigen
B cell epitope mapping
Escherichia coli LamB protein
Chagas disease
Language English
Date 1998-07-01
Published in Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 164, n. 1, p. 125-131, 1998.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 125-131
Origin http://dx.doi.org/10.1111/j.1574-6968.1998.tb13077.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000074571400019
URI http://repositorio.unifesp.br/handle/11600/25920

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