Effect of D-alpha-tocopherol on tubular nephron acidification by rats with induced diabetes mellitus

Effect of D-alpha-tocopherol on tubular nephron acidification by rats with induced diabetes mellitus

Autor Gomes, G. Nascimento Autor UNIFESP Google Scholar
Barbosa, F.t. Autor UNIFESP Google Scholar
Radaeli, R.f. Autor UNIFESP Google Scholar
Cavanal, Maria de Fátima Autor UNIFESP Google Scholar
Aires, M. Mello Google Scholar
Gil, Frida Zaladek Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo The objective of the present study was to determine if treatment of diabetic rats with D-alpha-tocopherol could prevent the changes in glomerular and tubular function commonly observed in this disease. Sixty male Wistar rats divided into four groups were studied: control (C), control treated with D-alpha-tocopherol (C + T), diabetic (D), and diabetic treated with D-alpha-tocopherol (D + T). Treatment with D-alpha-tocopherol (40 mg/kg every other day, ip) was started three days after diabetes induction with streptozotocin (60 mg/kg, ip). Renal function studies and microperfusion measurements were performed 30 days after diabetes induction and the kidneys were removed for morphometric analyses. Data are reported as means ± SEM. Glomerular filtration rate increased in D rats but decreased in D + T rats (C: 6.43 ± 0.21; D: 7.74 ± 0.45; D + T: 3.86 ± 0.18 ml min-1 kg-1). Alterations of tubular acidification observed in bicarbonate absorption flux (JHCO3) and in acidification half-time (t/2) in group D were reversed in group D + T (JHCO3, C: 2.30 ± 0.10; D: 3.28 ± 0.22; D + T: 1.87 ± 0.08 nmol cm-2 s-1; t/2, C: 4.75 ± 0.20; D: 3.52 ± 0.15; D + T: 5.92 ± 0.19 s). Glomerular area was significantly increased in D, while D + T rats exhibited values similar to C, suggesting that the vitamin prevented the hypertrophic effect of hyperglycemia (C: 8334.21 ± 112.05; D: 10,217.55 ± 100.66; D + T: 8478.21 ± 119.81µm²). These results suggest that D-alpha-tocopherol is able to protect rats, at least in part, from the harmful effects of diabetes on renal function.
Palavra-chave Diabetes
D-alpha-tocopherol
Renal function
Nephropathy
Idioma Inglês
Data de publicação 2005-07-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 7, p. 1043-1051, 2005.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 1043-1051
Fonte http://dx.doi.org/10.1590/S0100-879X2005000700007
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000230829900007
SciELO S0100-879X2005000700007 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/2591

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