Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice

Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice

Author Costa, F. Google Scholar
Franchin, G. Google Scholar
Pereira-Chioccola, V. L. Google Scholar
Ribeirao, M. Google Scholar
Schenkman, S. Google Scholar
Rodrigues, M. M. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Dante Pazzanese Cardiol Estado São Paulo
Abstract Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, does not synthesize sialic acid, but expresses a trans-sialidase that catalyses the transfer of sialic acid from host glycoconjugates to the parasite surface. Several lines of evidence suggest that this enzyme is a virulence factor implicated in the establishment of infection. Here we studied whether immunization with a plasmid DNA containing a gene encoding for rite catalytic domain of the enzyme could elicit protective immunity against T. cruzi infection in mice. We observed that immunization with this plasmid DNA generated antibody and T-cell mediated immune responses. Antibodies recognized the native enzyme and inhibited its activity in vitro. Upon challenge with bloodstream trypomastigotes, immunized animals displayed reduced parasitemia and mortality. (C) 1998 Elsevier B.V. All rights reserved.
Keywords Trypanosoma cruzi
DNA immunization
Language English
Date 1998-05-01
Published in Vaccine. Oxford: Elsevier B.V., v. 16, n. 8, p. 768-774, 1998.
ISSN 0264-410X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 768-774
Origin http://dx.doi.org/10.1016/S0264-410X(97)00277-6
Access rights Closed access
Type Article
Web of Science ID WOS:000073725300002
URI http://repositorio.unifesp.br/handle/11600/25893

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account