Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice

Immunization with a plasmid DNA containing the gene of trans-sialidase reduces Trypanosoma cruzi infection in mice

Autor Costa, F. Google Scholar
Franchin, G. Google Scholar
Pereira-Chioccola, V. L. Google Scholar
Ribeirao, M. Google Scholar
Schenkman, S. Google Scholar
Rodrigues, M. M. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Dante Pazzanese Cardiol Estado São Paulo
Resumo Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, does not synthesize sialic acid, but expresses a trans-sialidase that catalyses the transfer of sialic acid from host glycoconjugates to the parasite surface. Several lines of evidence suggest that this enzyme is a virulence factor implicated in the establishment of infection. Here we studied whether immunization with a plasmid DNA containing a gene encoding for rite catalytic domain of the enzyme could elicit protective immunity against T. cruzi infection in mice. We observed that immunization with this plasmid DNA generated antibody and T-cell mediated immune responses. Antibodies recognized the native enzyme and inhibited its activity in vitro. Upon challenge with bloodstream trypomastigotes, immunized animals displayed reduced parasitemia and mortality. (C) 1998 Elsevier B.V. All rights reserved.
Palavra-chave Trypanosoma cruzi
DNA immunization
Idioma Inglês
Data de publicação 1998-05-01
Publicado em Vaccine. Oxford: Elsevier B.V., v. 16, n. 8, p. 768-774, 1998.
ISSN 0264-410X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 768-774
Fonte http://dx.doi.org/10.1016/S0264-410X(97)00277-6
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000073725300002
Endereço permanente http://repositorio.unifesp.br/handle/11600/25893

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