Structure and pharmacological activities of a sulfated xylofucoglucuronan from the alga Spatoglossum schroederi

Structure and pharmacological activities of a sulfated xylofucoglucuronan from the alga Spatoglossum schroederi

Autor Leite, E. L. Google Scholar
Medeiros, MGL Google Scholar
Rocha, HAO Google Scholar
Farias, GGM Google Scholar
Silva, L. F. da Google Scholar
Chavante, S. F. Google Scholar
Abreu, L. D. de Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Instituição Univ Fed Rio Grande Norte
Universidade Federal de São Paulo (UNIFESP)
Resumo An acidic polysaccharide of 21 kDa, containing fucose, xylose, glucuronic acid and sulfate, accounting for 20% of the total acidic polysaccharides present in the alga Spatoglossum schroederi, was purified by ion exchange and molecular sieving chromatography. It migrated as a single component in agarose gel electrophoresis prepared in five different buffer systems with pH ranging from 2.0 to 9.0. Chemical analyses, methylation studies, degradation with glycosidases and sulfatases prepared from the mollusc Tagelus gibbus, acid hydrolysis, C-13 and H-1 NMR and infra-red spectroscopy led to the proposal of the structure of the compound and the mode of action of the enzymes. the polymer is composed of a core of beta(1-3)glucuronic acid-containing oligosaccharide of 4.5 kDa with branches at C-4 of the fucose chains alpha(1-3)-linked. the fucose is mostly substituted at C-4 with sulfate groups and at C-2 with chains of beta(1-4) xylose which, in turn is also partially sulfated. the xylofucoglucuronan has low anticoagulant and no hemorrhagic activity but, as heparin, has a high activity in stimulating the synthesis of an antithrombotic heparan sulfate from vascular endothelial cells in culture. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
Palavra-chave xylofucoglucuronan structure
algae
acidic polysaccharides
antithrombotic activity
glycosidases
mollusc
Idioma Inglês
Data de publicação 1998-03-16
Publicado em Plant Science. Clare: Elsevier Sci Ireland Ltd, v. 132, n. 2, p. 215-228, 1998.
ISSN 0168-9452 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 215-228
Fonte http://dx.doi.org/10.1016/S0168-9452(98)00012-0
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000073658100010
Endereço permanente http://repositorio.unifesp.br/handle/11600/25879

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