New pathway of heparan sulphate degradation Iduronate sulphatase and N-sulphoglucosamine 6-sulphatase act on the polymer chain prior to depolymerisation by a N-sulpho-glucosaminidase and glycuronidases in the mollusc Tagelus gibbus

New pathway of heparan sulphate degradation Iduronate sulphatase and N-sulphoglucosamine 6-sulphatase act on the polymer chain prior to depolymerisation by a N-sulpho-glucosaminidase and glycuronidases in the mollusc Tagelus gibbus

Author Medeiros, MGL Google Scholar
Ferreira, TMPC Google Scholar
Leite, E. L. Google Scholar
Toma, L. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UFRN
Abstract It has previously been shown that in the mollusc Anomalocardia brasiliana the desulphation of chondroitin sulphate precedes its depolymerisation by beta-glucuronidase and beta-N-acetylgalactosaminidase (Sousa Jr. et al. J. Biol. Chem. 1990;265:20150-20155). This led us to investigate whether in molluscs, sulphatases also act on heparan sulphate before its depolymerisation by glycosidases. Radioactively labelled [S-35]heparan sulphate was extensively degraded by enzyme extracts prepared from the mollusc Tagelus gibbus. Several enzymes acting in concert degrade the compound to inorganic sulphate, glucosamine N-sulphate, N-acetylglucosamine-6 sulphate and other oligosaccharide products. These results indicate the presence of iduronate sulphatase, N-sulphoglucosamine 6-sulphatase alpha-N-sulphoglucosaminidase, beta-glucuronidase and alpha-L-iduronidase. the di- and mono-saccharide composition of the oligosaccharides were analysed with the aid of heparitinase II from Flavobacterium, heparimum. These analyses led to the characterisation of two sulphatases that act on the polymer chain removing sulphates from the C-2 position of iduronic acid residues and the C-6 position of the glucosamine moieties, respectively. the different enzymes were partially fractionated by ion exchange chromatography and molecular sieving. These results led to the proposition of a new pathway of degradation of heparan sulphate where sulphatases act directly on the polymer chain which is then depolymerised by several glycosidases. (C) 1998 Elsevier Science Inc. All rights reserved.
Keywords mollusc iduronate sulphatase
mollusc N-sulphoglucosaminidase
mollusc heparan sulphate degradation
mollusc glycuronidases
mollusc sulphatases
glycosaminoglycan degradation molluscs
endosulphatases
mollusc
Language English
Date 1998-03-01
Published in Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology. Oxford: Pergamon-Elsevier B.V., v. 119, n. 3, p. 539-547, 1998.
ISSN 0305-0491 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 539-547
Origin http://dx.doi.org/10.1016/S0305-0491(98)00015-7
Access rights Closed access
Type Article
Web of Science ID WOS:000075233500014
URI http://repositorio.unifesp.br/handle/11600/25874

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