Autor |
Medeiros, MGL
![]() Ferreira, TMPC ![]() Leite, E. L. ![]() Toma, L. ![]() Dietrich, C. P. ![]() Nader, H. B. ![]() |
Instituição | Universidade Federal de São Paulo (UNIFESP) UFRN |
Resumo | It has previously been shown that in the mollusc Anomalocardia brasiliana the desulphation of chondroitin sulphate precedes its depolymerisation by beta-glucuronidase and beta-N-acetylgalactosaminidase (Sousa Jr. et al. J. Biol. Chem. 1990;265:20150-20155). This led us to investigate whether in molluscs, sulphatases also act on heparan sulphate before its depolymerisation by glycosidases. Radioactively labelled [S-35]heparan sulphate was extensively degraded by enzyme extracts prepared from the mollusc Tagelus gibbus. Several enzymes acting in concert degrade the compound to inorganic sulphate, glucosamine N-sulphate, N-acetylglucosamine-6 sulphate and other oligosaccharide products. These results indicate the presence of iduronate sulphatase, N-sulphoglucosamine 6-sulphatase alpha-N-sulphoglucosaminidase, beta-glucuronidase and alpha-L-iduronidase. the di- and mono-saccharide composition of the oligosaccharides were analysed with the aid of heparitinase II from Flavobacterium, heparimum. These analyses led to the characterisation of two sulphatases that act on the polymer chain removing sulphates from the C-2 position of iduronic acid residues and the C-6 position of the glucosamine moieties, respectively. the different enzymes were partially fractionated by ion exchange chromatography and molecular sieving. These results led to the proposition of a new pathway of degradation of heparan sulphate where sulphatases act directly on the polymer chain which is then depolymerised by several glycosidases. (C) 1998 Elsevier Science Inc. All rights reserved. |
Assunto |
mollusc iduronate sulphatase
mollusc N-sulphoglucosaminidase mollusc heparan sulphate degradation mollusc glycuronidases mollusc sulphatases glycosaminoglycan degradation molluscs endosulphatases mollusc |
Idioma | Inglês |
Data | 1998-03-01 |
Publicado em | Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology. Oxford: Pergamon-Elsevier B.V., v. 119, n. 3, p. 539-547, 1998. |
ISSN | 0305-0491 (Sherpa/Romeo, fator de impacto) |
Editor | Elsevier B.V. |
Extensão | 539-547 |
Fonte |
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Direito de acesso | Acesso restrito |
Tipo | Artigo |
Web of Science | WOS:000075233500014 |
URI | http://repositorio.unifesp.br/handle/11600/25874 |
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