The complete sequence of four major structural proteins of African horse sickness virus serotype 6: evolutionary relationships within and between the orbiviruses

The complete sequence of four major structural proteins of African horse sickness virus serotype 6: evolutionary relationships within and between the orbiviruses

Autor Williams, C. F. Google Scholar
Inoue, T. Google Scholar
Lucus, A. M. Google Scholar
Zanotto, PMDA Google Scholar
Polly, R. Google Scholar
Instituição Univ Oxford
NERC
Univ Alabama
Universidade Federal de São Paulo (UNIFESP)
Resumo The amino acid sequences of four major capsid proteins of African horse sickness virus (serotype 6, AHSV-6) have been determined from analyses of cDNA clones representing the L2, L3, M6 and S7 RNA segments. the AHSV-6 L3 RNA segment has an open reading frame of 2715 base pairs and encodes the inner capsid protein VP3 which comprises 905 amino acids. the VP3 layer forms the subcore of the virion and is surrounded by the VP7 protein which is encoded by the S7 gene. the AHSV-6 S7 gene was found to be 1047 nucleotides in length with a coding capacity for the VP7 protein of 349 amino acids. These core proteins are encapsulated by the outer capsid proteins VP5 and VP2 which are encoded by the M6 and L2 genes respectively. the M6 gene of AHSV-6 was determined to be 1564 nucleotides in length and encoded a protein product of 504 amino acids while the L2 gene comprised 3203 nucleotides which encoded a predicted protein product of 1051 amino acids. Comparison of these four sequences with the care protein sequences of other serotypes of African horse sickness virus, Bluetongue virus which infects sheep, and Epizootic haemorrhagic disease virus of deer, demonstrated that despite the pathobiological properties and host range of these distinct orbiviruses, extreme conservation is evident within the capsid genes. Sequence analyses also suggested that the similarity levels between serogroups depict the structure and function of the individual capsid proteins. the data indicated that the evolution of the capsid genes of gnat transmitted orbiviruses is strongly influenced by functional and structural constraints. (C) 1998 Elsevier Science B.V. All rights reserved.
Idioma Inglês
Data de publicação 1998-01-01
Publicado em Virus Research. Amsterdam: Elsevier B.V., v. 53, n. 1, p. 53-73, 1998.
ISSN 0168-1702 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 53-73
Fonte http://dx.doi.org/10.1016/S0168-1702(97)00131-7
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000073372000005
Endereço permanente http://repositorio.unifesp.br/handle/11600/25841

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