Vitamin D receptor gene polymorphism: Correlation with bone mineral density in a Brazilian population with insulin-dependent diabetes mellitus

Vitamin D receptor gene polymorphism: Correlation with bone mineral density in a Brazilian population with insulin-dependent diabetes mellitus

Author Hauache, O. M. Google Scholar
Lazaretti-Castro, M. Google Scholar
Andreoni, S. Google Scholar
Gimeno, SGA Google Scholar
Brandao, C. Google Scholar
Ramalho, A. C. Google Scholar
Kasamatsu, T. S. Google Scholar
Kunii, I Google Scholar
Hayashi, L. F. Google Scholar
Dib, S. A. Google Scholar
Vieira, JGH Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Patients with insulin-dependent diabetes mellitus (IDDM) are at higher risk of developing osteoporosis. Among the genetic factors related to die development of osteoporosis, a possible association between vitamin D receptor (VDR) gene polymorphism and bone mineral density (BMD) has been described in some populations. We characterized the VDR gene polymorphism in a healthy adult Brazilian population and in a group of patients with IDDM and correlated these findings with densitometric values in both groups. the Brazilian population is characterized by an important racial heterogeneity and therefore is considered an ethnically heterogeneous population. We recruited 94 healthy adult Brazilian volunteers (63 women and 31 men), mean (+/- SD) age 32.4 +/- 6.5 years (range 18-39 years), and 78 patients with IDDM (33 women and 45 men) diagnosed before 18 years of age, mean (+/- SD) age 23.3 +/- 5.5 years (range 18-39 years). VDR genotype was assessed by polymerase chain reaction amplification followed by BsmI digestion on DNA isolated from peripheral blood leukocytes. Statistical analysis included Bonferroni t-test to compare densitometric values within different genotypes in bath groups and multiple regression analysis of bone density adjusted for potential confounding factors. the IDDM group had a lower BMD compared with the control group. the VDR genotype distribution in the control group was 43 Bb (45.7%), 39 bb (41.5%) and 12 BB (12.8%). This distribution did not differ from that observed in the IDDM group: 39 Bb (50%), 26 bb (33.3%) and 13 BB (16.7%). in the IDDM group, patients with the Bb genotype had a higher body weight when compared with the BB genotype (p = 0.02). However, when diabetic patients were controlled for age, sea and body mass index, BB genotype was associated with a lower mean BMD at lumbar spine and femoral neck than in Bb and bb patients. BE patients had a shorter duration of IDDM than bb and Bb patients. These findings suggest a small influence of VDR gene polymorphism on BRID of a racially heterogeneous population with IDDM.
Keywords chronic complications
insulin-dependent diabetes mellitus
vitamin D
receptor gene polymorphism
Language English
Date 1998-01-01
Published in Osteoporosis International. Godalming: Springer-verlag London Ltd, v. 8, n. 3, p. 204-210, 1998.
ISSN 0937-941X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 204-210
Access rights Closed access
Type Article
Web of Science ID WOS:000078845100002

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