Determination of antipyrine and metabolites in plasma of a patient with mild renal failure

Determination of antipyrine and metabolites in plasma of a patient with mild renal failure

Autor Lanchote, V. L. Google Scholar
Ping, W. C. Google Scholar
Santos, SRCJ Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo A method has been developed for the simultaneous determination of antipyrine and its three major metabolites in plasma of patients with renal failure. Plasma samples (500 mu l) were hydrolyzed with beta-glucuronidase/aryl sulphatase. the compounds, after addition of sodium chloride, were extracted with chloroform:ethanol (90:10, v/v) in acidic medium. Chromatographic conditions comprise a C-18,, column, a mobile phase with 30% methanol and 70% 0.25N sodium acetate buffer (pH 5.0), a total run time of 10 minutes, and ultraviolet absorbance detection at 254 nm. Confidence limits showed 0.5 to 40.0 mu g/ml(-1) linearity (r(2) = 0.999); 0.1 mu g/ml(-1) HMA, 0.05 mu g/ml(-1) antipyrine and NORA, and 0.5 mu g/ml(-1) OHA sensitivity and absolute recovery >95%. Interprecision and intraprecision expressed as coefficient of variation were <10% for all compounds investigated. the assay shows to be suitable for pharmacokinetics and drug metabolism studies after administration of a single oral dose of 500 mg of antipyrine to a patient with hypertension and chronic renal failure (CLCR = 34.17 ml/min(-1); 1.73m(-2)).
Palavra-chave HPLC method
antipyrine metabolites
plasma assay
renal failure
pharmacokinetics
Idioma Inglês
Data de publicação 1997-12-01
Publicado em Therapeutic Drug Monitoring. Philadelphia: Lippincott-raven Publ, v. 19, n. 6, p. 705-710, 1997.
ISSN 0163-4356 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott-raven Publ
Extensão 705-710
Fonte http://dx.doi.org/10.1097/00007691-199712000-00017
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997YK57100017
Endereço permanente http://repositorio.unifesp.br/handle/11600/25826

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