Renal expression of two rat kallikrein genes under diabetic conditions

Renal expression of two rat kallikrein genes under diabetic conditions

Author Tschope, C. Google Scholar
Yu, M. H. Google Scholar
Reinecke, A. Google Scholar
Csikos, T. Google Scholar
Hilgenfeldt, U. Google Scholar
Pesquero, J. B. Google Scholar
Ritz, E. Google Scholar
Unger, T. Google Scholar
Institution Free Univ Berlin
Univ Heidelberg
Univ Kiel
Universidade Federal de São Paulo (UNIFESP)
Abstract Objective We have reported that bradykinin (BK) excretion is increased in severely diabetic rats, independent of the activity of the main renal kinin-forming enzyme, true kallikrein (KLK). To further investigate the relationship between renal BK excretion and renal KLK in diabetes we studied the regulation of the renal kallikrein-like gene, rat kallikrein 7 (rKLK7), as well as of the KLK encoding gene, rKLK1, in streptozotocin-induced (STZ) diabetic rats.Methods Experiments were performed in STZ-induced diabetic male Wistar rats and their non-diabetic controls (n = 7 each group). Twelve weeks after STZ injection, urinary KLK activity, glomerular filtration rate and total protein excretion were determined. After extraction of total renal cortical RNA, specific oligonucleotides were used to generate a reverse transcription-polymerase chain reaction (RT-PCR) products of renal cortical rKLK1 and rKLK7 messenger (m) RNA. Southern blot analysis of these RT-PCR products were hybridized with appropriate gene-specific oligonucleotide probes.Results After 12 weeks, the rats showed hyperglycemia, proteinuria and a reduced glomerular filtration rate. Renal kininogenase was reduced, as indicated by a reduction in the expression of rKLK1, as well as of the KLK-related gene, rKLK7.Conclusions Our data show that the expression of the two principal renal KLK genes is downregulated in the renal cortex of STZ-diabetic rats. We suggest that under severe diabetic conditions the rise in urinary BK excretion is not related to activation of the renal kinin-forming enzyme system. (C) Rapid Science Publishers ISSN 0263-6352.
Keywords kallikrein
diabetic nephropathy
esterase B
Language English
Date 1997-12-01
Published in Journal of Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 15, n. 12, p. 1711-1714, 1997.
ISSN 0263-6352 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 1711-1714
Access rights Closed access
Type Article
Web of Science ID WOS:000071663700023

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