Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops

Culture supernatant of Shiga toxin-producing Escherichia coli strains provoke fluid accumulation in rabbit ileal loops

Autor Ferreira, Antonio José Piantino Google Scholar
Elias Junior, Waldir Pereira Autor UNIFESP Google Scholar
Pelayo, Jacinta Sanchez Google Scholar
Giraldi, Regina Autor UNIFESP Google Scholar
Pedroso, Margareth Zabeu Autor UNIFESP Google Scholar
Scaletsky, Isabel Cristina Affonso Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Universidade Estadual de Londrina (UEL)
Resumo Production of Shiga toxin (Stx) in Escherichia coli strains belonging to serogroups O26, O111, and O157 was evaluated in the rabbit ileal loop assay and results were compared to those using tissue culture assays and DNA hybridization with specific probes for Stx1 and Stx2. All 14 Shiga toxin-producing E. coli strains tested provoked fluid accumulation in the rabbit intestinal loop. Eleven strains hybridized with Stx1 probe, one strain with Stx2 and two strains with both probes. Filtered culture supernatants of all E. coli strains presented cytotoxic effects in both HeLa and Vero cells, in this study, we found a strong association between the production of Stx and its effect in an animal model. This is the first description of high-level Stx-producing E. coli O111ac isolated in Brazil. (C) 1998 Published by Elsevier Science B.V. All rights reserved.
Palavra-chave Escherichia coli
shiga-like toxin
rabbit ileal loop assay
Idioma Inglês
Data de publicação 1997-12-01
Publicado em Fems Immunology and Medical Microbiology. Hoboken: Wiley-Blackwell, v. 19, n. 4, p. 285-288, 1997.
ISSN 0928-8244 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 285-288
Fonte http://dx.doi.org/10.1111/j.1574-695X.1997.tb01098.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000071594600004
Endereço permanente http://repositorio.unifesp.br/handle/11600/25814

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