Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener

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dc.contributor.author Zingales, B.
dc.contributor.author Pereira, MES
dc.contributor.author Oliveira, R. P.
dc.contributor.author Almeida, K. A.
dc.contributor.author Umezawa, E. S.
dc.contributor.author Souto, R. P.
dc.contributor.author Vargas, N.
dc.contributor.author Cano, M. I.
dc.contributor.author daSilveira, J. F.
dc.contributor.author Nehme, N. S.
dc.contributor.author Morel, C. M.
dc.contributor.author Brener, Z.
dc.contributor.author Macedo, A.
dc.date.accessioned 2016-01-24T12:30:28Z
dc.date.available 2016-01-24T12:30:28Z
dc.date.issued 1997-11-01
dc.identifier http://dx.doi.org/10.1016/S0001-706X(97)00088-0
dc.identifier.citation Acta Tropica. Amsterdam: Elsevier B.V., v. 68, n. 2, p. 159-173, 1997.
dc.identifier.issn 0001-706X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/25804
dc.description.abstract Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. CL Brener was obtained by cloning procedures from bloodstream trypomastigotes isolated from mice infected with the CL strain. the doubling time of CL Brener epimastigotes cultured at 28 degrees C in liver infusion-tryptose (LIT) medium is 58 +/- 13 h. Differentiation to metacyclic forms is induced by incubation of epimastigotes in LIT-20% Grace's medium. Metacyclics give very low parasitemia in mice, contrary to what is observed for blood forms which promote 100% mortality of the animals with inocula of 5 x 10(3) parasites. CL Brener blood forms are highly susceptible to nifurtimox, benznidazole and ketoconazole. Allopurinol is inefficient in the treatment of mice experimental infection. the clone infects mammalian cultured cells and performs the complete intracellular cycle at 33 and 37 degrees C. the molecular typing of CL Brener has been done by isoenzymatic profiles; sequencing of a 24S alpha ribosomal RNA gene domain and by schizodeme, randomly amplified polymorphic DNA and DNA fingerprinting analyses. for each typing approach the patterns obtained do not change after prolonged parasite subcultivation in LIT medium (up to 100 generations). the stability of the molecular karyotype of the clone was also confirmed. (C) 1997 Elsevier Science B.V. en
dc.format.extent 159-173
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Acta Tropica
dc.rights Acesso restrito
dc.subject Trypanosoma cruzi en
dc.subject Genome project en
dc.subject reference organism en
dc.subject molecular typing en
dc.subject biological characteristics en
dc.title Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution FIOCRUZ MS
dc.contributor.institution Universidade Federal de Minas Gerais (UFMG)
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation FIOCRUZ MS,CTR PESQUISAS RENE RACHOU,BELO HORIZONT,MG,BRAZIL
dc.description.affiliation UFMG,INST CIENCIAS BIOL,BELO HORIZONT,MG,BRAZIL
dc.description.affiliation UNIV São Paulo,INST TROP MED,São Paulo,BRAZIL
dc.description.affiliation UNIFESP,ESCOLA PAULISTA MED,São Paulo,BRAZIL
dc.description.affiliation FIOCRUZ MS,INST OSWALDO CRUZ,BR-21045900 RIO JANEIRO,BRAZIL
dc.description.affiliationUnifesp UNIFESP,ESCOLA PAULISTA MED,São Paulo,BRAZIL
dc.identifier.doi 10.1016/S0001-706X(97)00088-0
dc.description.source Web of Science
dc.identifier.wos WOS:A1997YC38200003



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