Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener

Trypanosoma cruzi genome project: biological characteristics and molecular typing of clone CL Brener

Author Zingales, B. Google Scholar
Pereira, MES Google Scholar
Oliveira, R. P. Google Scholar
Almeida, K. A. Google Scholar
Umezawa, E. S. Google Scholar
Souto, R. P. Google Scholar
Vargas, N. Google Scholar
Cano, M. I. Google Scholar
daSilveira, J. F. Google Scholar
Nehme, N. S. Google Scholar
Morel, C. M. Google Scholar
Brener, Z. Google Scholar
Macedo, A. Google Scholar
Institution FIOCRUZ MS
Universidade Federal de Minas Gerais (UFMG)
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. CL Brener was obtained by cloning procedures from bloodstream trypomastigotes isolated from mice infected with the CL strain. the doubling time of CL Brener epimastigotes cultured at 28 degrees C in liver infusion-tryptose (LIT) medium is 58 +/- 13 h. Differentiation to metacyclic forms is induced by incubation of epimastigotes in LIT-20% Grace's medium. Metacyclics give very low parasitemia in mice, contrary to what is observed for blood forms which promote 100% mortality of the animals with inocula of 5 x 10(3) parasites. CL Brener blood forms are highly susceptible to nifurtimox, benznidazole and ketoconazole. Allopurinol is inefficient in the treatment of mice experimental infection. the clone infects mammalian cultured cells and performs the complete intracellular cycle at 33 and 37 degrees C. the molecular typing of CL Brener has been done by isoenzymatic profiles; sequencing of a 24S alpha ribosomal RNA gene domain and by schizodeme, randomly amplified polymorphic DNA and DNA fingerprinting analyses. for each typing approach the patterns obtained do not change after prolonged parasite subcultivation in LIT medium (up to 100 generations). the stability of the molecular karyotype of the clone was also confirmed. (C) 1997 Elsevier Science B.V.
Keywords Trypanosoma cruzi
Genome project
reference organism
molecular typing
biological characteristics
Language English
Date 1997-11-01
Published in Acta Tropica. Amsterdam: Elsevier B.V., v. 68, n. 2, p. 159-173, 1997.
ISSN 0001-706X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 159-173
Access rights Closed access
Type Article
Web of Science ID WOS:A1997YC38200003

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