Activities of monoamine oxidase (MAO) A and B in discrete regions of rat brain after rapid eye movement (REM) sleep deprivation

Activities of monoamine oxidase (MAO) A and B in discrete regions of rat brain after rapid eye movement (REM) sleep deprivation

Autor Perez, N. M. Google Scholar
Venditti, Marco Antonio Campana Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Rapid eye movement (REM) sleep deprivation increases monoaminergic (noradrenergic and serotonergic) turnover and their metabolites in whole brain of rats. This increase in metabolites may indicate increased activity of the enzymes responsible for the inactivation of monoamines. To test this hypothesis, we assayed the activity of monoamineoxidases (MAOs) A and B in hippocampus, hypothalamus, brainstem and its divisions pens and medulla oblongata in rats deprived of REM sleep for 96h. REM sleep deprivation was carried out by the newer-pot technique. A control group remained in their home cages. MAO A was assayed by using [C-14]-5-hydroxytryptamine as the substrate (50 mu M final concentration) and MAO B by using [C-14]-beta-phenylethylamine (2 mu M final concentration). the enzymes were assayed in the mitochondrial fraction. the results obtained showed that a significant decrease in the activity of MAO A was obtained in the brainstem and an increase in medulla oblongata and no statistical differences in the activity of MAO B in brainstem, pens and medulla oblongata and MAO Pr in pons; there were also no differences in the activities of both MAO A and B in hippocampus and hypothalamus. Although our results confirmed previous data regarding changes in MAO A activity in brainstem and medulla oblongata, they did not confirm our hypothesis that the increase in monamine turnover and metabolites in the brain would be the result of increased MAO activity. (C) 1997 Elsevier Science Inc.
Palavra-chave REM sleep deprivation
monoamine oxidases
brain regions
Idioma Inglês
Data de publicação 1997-10-01
Publicado em Pharmacology Biochemistry and Behavior. Oxford: Pergamon-Elsevier B.V., v. 58, n. 2, p. 605-608, 1997.
ISSN 0091-3057 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 605-608
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997XU25900044
Endereço permanente

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