Determination of 5-aminolevulinic acid in blood plasma, tissues and cell cultures by high-performance liquid chromatography with electrochemical detection

Determination of 5-aminolevulinic acid in blood plasma, tissues and cell cultures by high-performance liquid chromatography with electrochemical detection

Autor Costa, C. A. Google Scholar
Trivelato, G. C. Google Scholar
Demasi, M. Google Scholar
Bechara, EJH Google Scholar
Instituição Universidade de São Paulo (USP)
FDN JORGE DUPRAT FIGUEIREDO & MED TRABALHO
Universidade Federal de São Paulo (UNIFESP)
Resumo A simple and sensitive method for determining 5-aminolevulinic acid (ALA) in biological samples is described. ALA is derivatized with o-phthaldehyde to give a compound with favorable properties for high-performance Liquid chromatography with electrochemical detection. the method does not require extensive pretreatment of the samples and its detection limit is in the range of 1 pmol/20 mu l injection. This method was applied to the determination of plasma ALA from normal and lead-exposed subjects, where 0.26 +/- 0.08 mu M (n = 30) and 2.6 +/- 0.75 mu M (n = 30), respectively were found. We also determined ALA in rat tissues, namely liver and brain, and the uptake of ALA by cultured fibroblasts and hepatocytes to illustrate the diversified applicability of the method. (C) 1997 Elsevier Science B.V.
Palavra-chave 5-aminolevulinic acid
Idioma Inglês
Data de publicação 1997-08-01
Publicado em Journal of Chromatography B. Amsterdam: Elsevier B.V., v. 695, n. 2, p. 245-250, 1997.
ISSN 0378-4347 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 245-250
Fonte http://dx.doi.org/10.1016/S0378-4347(97)00139-4
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997XR48500006
Endereço permanente http://repositorio.unifesp.br/handle/11600/25772

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