Cloning and characterization of a gene encoding a novel immunodominant antigen of Trypanosoma cruzi

Show simple item record Lesenechal, M. Duret, L. Cano, M. I. Mortara, R. A. Jolivet, M. Camargo, M. E. daSilveira, J. F. ParanhosBaccala, G. 2016-01-24T12:30:24Z 2016-01-24T12:30:24Z 1997-08-01
dc.identifier.citation Molecular and Biochemical Parasitology. Amsterdam: Elsevier B.V., v. 87, n. 2, p. 193-204, 1997.
dc.identifier.issn 0166-6851
dc.description.abstract A Trypanosoma cruzi genomic expression library was screened with a pool of sera obtained from chronic chagasic patients. the recombinant antigen (Tc40) isolated from this library reacted with a large number of serum samples of chronic chagasic patients, suggesting that the presence of anti-Tc40 antibodies may be specifically associated to Chagas' disease. the full-length sequence of the Tc40 gene was determined after isolation of genomic and cDNA clones. the Tc40 cDNA includes a large open reading frame (2745 bp-long) that encodes a polypeptide of 100 kDa without any homology with previously described T. cruzi sequences. in contrast with other T. cruzi antigens whose immunodominant B-cell epitopes are composed by amino acid repetitive motifs, Tc40 does not show any amino acid repetition. Antibodies against the Tc40 recombinant protein reacted with three native polypeptides of 100, 41 and 38 kDa which are lightly associated with membranes or cytoskeleton and expressed in all developmental stages of the parasite life cycle. A transcript of 3.9-kb was detected in Northern blot analysis which is large enough to encode a 100 kDa polypeptide. Tc40 genes were mapped on a chromosomal band of 1.1 Mbp and in a few copies per haploid genome in the G strain. (C) 1997 Elsevier Science B.V. en
dc.format.extent 193-204
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Molecular and Biochemical Parasitology
dc.rights Acesso restrito
dc.subject cloning en
dc.subject immunodominant antigen en
dc.subject T-cruzi en
dc.title Cloning and characterization of a gene encoding a novel immunodominant antigen of Trypanosoma cruzi en
dc.type Artigo
dc.contributor.institution ECOLE NORMALE SUPER LYON
dc.contributor.institution LAB BIOMETRIE
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution BIOLAB MERIEUX
dc.description.affiliation LAB BIOMETRIE,CNR,URA 2055,VILLEURBANNE,FRANCE
dc.description.affiliation ESCOLA PAULISTA MED,DEPT PARASITOL,BR-04023 São Paulo,BRAZIL
dc.description.affiliation BIOLAB MERIEUX,São Paulo,BRAZIL
dc.description.affiliationUnifesp ESCOLA PAULISTA MED,DEPT PARASITOL,BR-04023 São Paulo,BRAZIL
dc.identifier.doi 10.1016/S0166-6851(97)00068-6
dc.description.source Web of Science
dc.identifier.wos WOS:A1997XL35000007


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