Cloning, structural analysis and expression of the gene encoding Hsp32 from Dictyostelium discoideum

Cloning, structural analysis and expression of the gene encoding Hsp32 from Dictyostelium discoideum

Autor DeMaria, A. C. Google Scholar
Moerman, A. Google Scholar
Klein, C. Google Scholar
Gomes, S. L. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo We have isolated and characterized genomic clones encoding a novel small heat shock (HS) protein (Hsp32) from Dictyostelium discoideum that is not homologous to the a-crystallin family Hsps. Besides its induction by HS, this gene is also regulated during the life cycle of the organism. At physiologic temperatures hsp32 is expressed at high levels in growing cells and at low levels in cells starved to initiate their developmental programme. However, in both cases the gene can be induced by HS. A DNA fragment containing the upstream region of hsp32 was shown to confer HS induction to a cat reporter gene, indicating a transcriptional regulation for this gene. A single transcription start site, located at position -152 relative to the initiator Met, 17 nucleotides downstream from a putative TATA box, was determined both in vegetative cells and cells starved for 6 h. This site was unchanged when either vegetative or starved cells were submitted to HS at 30 degrees C for 30 min. Despite HS induction, a perfect HSE element was not found in the 5' regulatory region of the gene. the hsp32 coding region is interrupted by a single intron located near its 5' end, which is properly spliced even under HS conditions. (C) 1997 Elsevier Science B.V.
Assunto heat shock protein
gene structure
intron splicing
developmental expression
Idioma Inglês
Data 1997-07-09
Publicado em Gene. Amsterdam: Elsevier B.V., v. 193, n. 2, p. 173-180, 1997.
ISSN 0378-1119 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 173-180
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997XM28100006

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