Distribution of epitopes of Trypanosoma cruzi amastigotes during the intracellular life cycle within mammalian cells

Distribution of epitopes of Trypanosoma cruzi amastigotes during the intracellular life cycle within mammalian cells

Autor Barros, H. C. Google Scholar
Verbisck, N. V. Google Scholar
DaSilva, S. Google Scholar
Araguth, M. F. Google Scholar
Mortara, R. A. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo In this study we have examined the distribution of epitopes defined by monoclonal antibodies raised against Trypanosoma cruzi amastigotes during the intracellular life cycle of the parasite. We have raised monoclonal antibodies towards amastigote forms and performed preliminary immunochemical characterization of their reactivities. MAB 1D9, 3G8, 2B7, 3B9, and 4B9 react with carbohydrate epitopes of the parasite major surface glycoprotein-Ssp-4 defined by MAB 2C2 [5]; MAB 4B5 reacts with a noncarbohydrate epitope in all developmental stages of the parasite, and MAB 3B2 also detects a noncarbohydrate epitope preferentially in T. cruzi flagellated forms. Vero cells infected with tissue culture-derived trypomastigotes of clone D11 (G strain) were fixed at different times during the intracellular proliferation of parasites, and processed for immune-electron microscopy and confocal immunofluorescence with the different monoclonal antibodies. We observed that while the surface distribution of MAB 2C2 and 4B9 epitopes was uniform throughout the cycle, MAB 1D9, 3G8, and 2B7 reacted with cytoplasmic membrane-bound compartments of the amastigotes. MAB 3B9 displayed a unique surface dentate pattern in some amastigotes. MAB 4B5 recognized a curved-shaped structure at the flagellar pocket region in some intracellular amastigotes and localized to the membrane in dividing forms. in intracellular trypomastigotes, MAB 4B5 also displayed a punctate pattern near the flagellar pocket.
Palavra-chave amastigote antigens
confocal microscopy
immuno-electron microscopy
monoclonal antibodies
Idioma Inglês
Data de publicação 1997-07-01
Publicado em Journal of Eukaryotic Microbiology. Lawrence: Soc Protozoologists, v. 44, n. 4, p. 332-344, 1997.
ISSN 1066-5234 (Sherpa/Romeo, fator de impacto)
Publicador Soc Protozoologists
Extensão 332-344
Fonte http://dx.doi.org/10.1111/j.1550-7408.1997.tb05675.x
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997XJ82800009
Endereço permanente http://repositorio.unifesp.br/handle/11600/25761

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