DNA antisense strategies in the study of receptors for vasoactive peptides, and of growth and wound-healing factors

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dc.contributor.author DOrleansJuste, P.
dc.contributor.author Sirois, M. G.
dc.contributor.author Edelman, E. R.
dc.contributor.author Regoli, D.
dc.contributor.author Pheng, L. H.
dc.contributor.author Bkaily, G.
dc.contributor.author Lindsey, C. J.
dc.date.accessioned 2016-01-24T12:30:23Z
dc.date.available 2016-01-24T12:30:23Z
dc.date.issued 1997-07-01
dc.identifier http://dx.doi.org/10.1023/A:1006800629012
dc.identifier.citation Molecular and Cellular Biochemistry. Dordrecht: Kluwer Academic Publ, v. 172, n. 1-2, p. 199-211, 1997.
dc.identifier.issn 0300-8177
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/25759
dc.description.abstract Antisense oligodeoxynucleotide technology has contributed greatly to the overall understanding of both mRNA stability as well as translational processes leading to protein synthesis. Arrest of translational processes by DNA antisense strands usually reduces maximal effects of agonists without affecting their apparent affinities in treated isolated vascular or nonvascular preparations.In the present study, examples are given of DNA antisense oligonucleotide-induced repression of receptors for endothelins, kinins as well as of the platelet-derived growth factor. Furthermore, the efficiency of this technology illustrates the roles of protooncogenes (c-myc and c-myb) in wound-healing mechanisms.The overall mechanism of action of these oligomers is described and the relevance of size, chemical alterations and mode of delivery are illustrated. Release of oligophosphorothioates from polymer matrices and gels can produce a prolonged effect in vivo. Antisense oligonucleotides remain essential in experimental models for which receptor antagonists or selective inhibitors of intracellular components are currently unavailable. en
dc.format.extent 199-211
dc.language.iso eng
dc.publisher Kluwer Academic Publ
dc.relation.ispartof Molecular and Cellular Biochemistry
dc.rights Acesso restrito
dc.subject DNA antisense en
dc.subject oligophosphodiester en
dc.subject oligophosphorothioate en
dc.subject ETA and B-1 receptors en
dc.subject c-myc and c-myb protooncogenes en
dc.subject PDGF-beta receptor subunit en
dc.title DNA antisense strategies in the study of receptors for vasoactive peptides, and of growth and wound-healing factors en
dc.type Artigo
dc.contributor.institution UNIV SHERBROOKE
dc.contributor.institution HARVARD UNIV
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNIV SHERBROOKE,FAC MED,DEPT ANAT & CELLULAR BIOL,SHERBROOKE,PQ J1H 5N4,CANADA
dc.description.affiliation HARVARD UNIV,MIT,DIV HLTH SCI & TECHNOL,CAMBRIDGE,MA 02139
dc.description.affiliation Universidade Federal de São Paulo,DEPT BIOPHYS,ESCOLA PAULISTA MED,BR-04023062 São Paulo,SP,BRAZIL
dc.description.affiliationUnifesp Universidade Federal de São Paulo,DEPT BIOPHYS,ESCOLA PAULISTA MED,BR-04023062 São Paulo,SP,BRAZIL
dc.identifier.doi 10.1023/A:1006800629012
dc.description.source Web of Science
dc.identifier.wos WOS:A1997XU44700021



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