Isolation and characterization of a new clotting factor from Bothrops jararacussu (Jararacucu) venom

Isolation and characterization of a new clotting factor from Bothrops jararacussu (Jararacucu) venom

Autor AndriaoEscarso, S. H. Google Scholar
Sampaio, S. V. Google Scholar
Cunha, OAB Google Scholar
Marangoni, S. Google Scholar
Oliveira, B. Google Scholar
Giglio, JR Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Resumo A detailed procedure for the isolation of a new clotting enzyme from the venom of Bothrops jararacussu (common name jararacucu) is described. the estimated mol. wt of the native protein was 30,100 but 37,500 after reduction by dithiothreitol. Two major close bands corresponding to pI 5.18 and 5.20 were detected by electrofocusing but, after methanolysis, a single band focused at pI 8.20, the mel. wt of the protein moiety of this glycoprotein was 28,500, showing V-V-G-A-D-N-C-N-F-N... as N-terminal sequence. the content of neutral sugar was 4.8% and that of total sugars 5.3%. This clotting factor degraded only the A alpha-chain of the fibrinogen molecule. the stability of the clot, when produced in the presence of aprotinin, opens new uses for snake clotting enzymes in the production of fibrin glue. (C) 1997 Elsevier B.V.
Idioma Inglês
Data de publicação 1997-07-01
Publicado em Toxicon. Oxford: Pergamon-Elsevier B.V., v. 35, n. 7, p. 1043-1052, 1997.
ISSN 0041-0101 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 1043-1052
Fonte http://dx.doi.org/10.1016/S0041-0101(96)00222-X
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:A1997XK24300006
Endereço permanente http://repositorio.unifesp.br/handle/11600/25755

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